Tetracycline-inducible Expression Systems:New Strategies and Practices in the Transgenic Mouse Modeling  

作　　者：Yan SUN Xigu CHEN Dong XIAO 

机构地区：[1]Center of Experimental Animals, Sun Yat-Sen University Guangzhou 510080, China [2]Institute of Comparative Medicine and Center of Experimental Animals, Southern Medical Universi~_ , Guangzhou 510515, China

出　　处：《Acta Biochimica et Biophysica Sinica》2007年第4期235-246,共12页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(No.30271177 and 39870676);the National 9th Five-year Program(No.101033);the Major Science and Technology Projects of Guangdong Province(No.B602);the Natural Science Foundation of Guangdong Province(No.021903);the Postdoctoral Fellowship Foundation of China(Series 29);the Special Fund of Scientific Instrument Collaborative Share-net in Guangzhou(No.2006176)

摘　　要：To accurately analyze the function of transgene（s） of interest in transgenic mice, and to generate credible transgenic animal models for multifarious human diseases to precisely mimic human disease states, it is critical to tightly regulate gene expression in the animals in a conditional manner. The ability to tum gene expression on or off in the restricted cells or tissues at specific time permits unprecedented flexibility in dissecting gene functions in health and disease. Pioneering studies in conditional transgene expression have brought about the development of a wide variety of controlled gene expression systems, which meet this criterion. Among them, the tetracycline-controlled expression systems （e.g. Tet-off system and Tet-on system） have been used extensively in vitro and in vivo. In recent years, some strategies derived from tetracycline-inducible system alone, as well as the combined use of Tet-based systems and Crellox P switching gene expression system, have been newly developed to allow more flexibility for exploring gene functions in health and disease, and produce credible transgenic animal models for various human diseases. In this review these newly developed strategies are discussed.To accurately analyze the function of transgene（s） of interest in transgenic mice, and to generate credible transgenic animal models for multifarious human diseases to precisely mimic human disease states, it is critical to tightly regulate gene expression in the animals in a conditional manner. The ability to tum gene expression on or off in the restricted cells or tissues at specific time permits unprecedented flexibility in dissecting gene functions in health and disease. Pioneering studies in conditional transgene expression have brought about the development of a wide variety of controlled gene expression systems, which meet this criterion. Among them, the tetracycline-controlled expression systems （e.g. Tet-off system and Tet-on system） have been used extensively in vitro and in vivo. In recent years, some strategies derived from tetracycline-inducible system alone, as well as the combined use of Tet-based systems and Crellox P switching gene expression system, have been newly developed to allow more flexibility for exploring gene functions in health and disease, and produce credible transgenic animal models for various human diseases. In this review these newly developed strategies are discussed.

关 键 词：tetracycline-inducible expression system Crellox P system transgenic mouse modeling leaky expression stringent control lineage-specific gene expression lineage-specific RNAi 

分 类 号：Q786[生物学—分子生物学]