雌激素对血管平滑肌细胞凋亡及c-myc基因表达的影响  

Effect of estrogen on apoptosis and expression of c-myc protein in cultured vascular smooth muscle cells of rabbit

作　　者：周永兰[1] 陈清枝[2] 张延斌[2] 王人彭[1] 张义勤[1] 骆秉辁[1] 

机构地区：[1]江苏大学附属医院徐州市第三人民医院心内科,江苏徐州221005 [2]徐州医学院附属医院心内科

出　　处：《临床心血管病杂志》2007年第4期301-303,共3页Journal of Clinical Cardiology

摘　　要：目的探讨17β雌二醇(E2)对血管平滑肌细胞(VSMC)凋亡的影响及其可能机制。方法应用流式细胞仪检测低、高浓度17βE2(10、100nmol/L)对传代的VSMC凋亡率的影响,并通过细胞免疫组化方法观察对凋亡相关基因c-myc表达的影响。结果17βE2作用下VSMC凋亡率显著高于对照组(P<0.01),伴随着VSMC凋亡率的增加,c-myc蛋白的表达亦增强,且表现明显的浓度依赖性。结论17βE2具有诱导VSMC凋亡的作用,其部分机制可能是通过上调促凋亡基因c-myc的表达有关。Objective：To investigate the effects of 17β estradiol （17β-E2） on apoptosis of vascular smooth muscle cells （VSMC） and the possible underlying mechanism. Method： The apoptosis was examined by flow cytometry in subcultured VSMC exposed to different concentrations （10, 100 nmol/L）of 17β-E2. The intracellular localization of c-myc protein in VSMC was detected by immunocytochemical method. Result： The ratio of VSMCs apoptosis was increased significantly by 17β-E2 with dose-dependant compared with control group（P〈0.01）, as accompanied by a significant raise of pro-apoptosis c-myc protein expression. Conclusion： 17β-E2 might induce the apoptosis of VSMC in part by the reason that the expression of pro-apoptosis oncogene c-myc protein upregulates.

关 键 词：雌二醇 血管平滑肌细胞 细胞凋亡 c—myc蛋白 

分 类 号：R977.1[医药卫生—药品]