经液氮保存有活性同种带支架瓣膜的实验研究  

Experimental Study of Viable Stented Homograft Valve Preserved in Liquid Nitrogen

作　　者：李志强[1] 刘迎龙[1] 朱晓东[1] 谢宁[1] 

机构地区：[1]中国医学科学院中国协和医科大学阜外心血管病医院先心病研究室,北京100037

出　　处：《中国胸心血管外科临床杂志》2007年第2期112-116,共5页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：国家高科技研究发展计划(863计划;2001AA216061)~~

摘　　要：目的检测经液氮保存的人同种带支架瓣膜的细胞活性和流体力学性能。方法制作同种带支架瓣膜经液氮保存3个月后,利用流式细胞仪技术定量检测瓣叶细胞活性(实验组,n=6),采集新鲜同种瓣膜作为对照(对照组,n=6)。利用国产脉动流实验装置,分别测试实验组21#、23#、25#同种带支架瓣膜的流体力学性能,测试各流量下的跨瓣压差、有效瓣口面积和回流百分比;并使用相应型号的国产Perfect牛心包生物瓣膜进行对比研究。结果实验组内皮细胞死亡率与对照组比较差异无统计学意义(10.24%±1.71%vs.9.09%±2.72%,P=0.441);实验组平滑肌细胞死亡率与对照组比较差异无统计学意义(8.76%±1.82%vs.7.84%±0.59%,P=0.178);实验组总细胞死亡率与对照组比较差异无统计学意义(8.79%±1.44%vs.7.40%±0.49%,P=0.072)。两种21#、23#、25#人工生物瓣膜的跨瓣压差对流量具有非常大的依赖性,随着流量的增大而增大。同种带支架瓣膜的跨瓣压差较Perfect牛心包生物瓣膜的大。两种各型号瓣膜的回流百分比均随测试流量的增大而略有减小,同样,同种带支架瓣膜的回流百分比较Perfect牛心包生物瓣膜的大。两种各型号瓣膜的有效瓣口面积均有随流量增加而增大的趋势,同种带支架瓣膜的有效瓣口面积较Perfect牛心包生物瓣膜的略小。结论经液氮保存同种带支架瓣膜的细胞活性保存良好,流体力学性能较满意。Objective To examine the cell viability and hemodynamic functions of the stented homograft valves preserved in liquid nitrogen. Methods Cell viability of the stented homograft valve preserved in liquid nitrogen after 3 months of preservation （experimental group,n = 6） was examined using flow cytometer. Fresh homografts served as control group （n= 6）. We prepared three sorts of stented homograft valve（21 # , 23 # , 25 # ） preserved by liquid nitrogen. In vitro pulsatile flow tests were performed on valves of two groups. Effective opening area （EOA）, transvalve pressure gradient and regurgitation ratio were recorded at various flow volume, and compare with Perfect bioprosthetic valve. Results The results revealed that the death ratio of endothelial cell was 10. 24% ±1.71% in the experimental group, and 9.09%±2.72% in the control group （P=0. 441）. The death ratio of smooth muscle cell was 8.76%±1.82% in the experimental group, and 7. 84%±0.59% （P=0. 178） in the control group. The death ratio of total cell was 8. 79%±1. 440.% in the experimental group, and 7. 40%±0. 49% in the control group （P=0.072）. There were no significantly differences between two groups. The transvalve pressure gradient of two groups of valve depended on the flow volume, and increased with the flow volume increasing. The transvalve pressure gradient of the stented bomograft valve was higher than that of Perfect valve. Regurgitation ratio of the stented homograft valve was bigger than Perfect valve＇ s. EOA had an increasing character when flow volume increased. EOA of the stented homograft valve was smaller than that of Perfect valve＇ s. Conclusion Liquid nitrogen can offer the benefit of cell viability of the stented homograft bioprosthetic valves. The stented homograft valve has salisfactory hemodynamic functions.

关 键 词：同种瓣 支架 液氮 细胞活性 流体力学 

分 类 号：R318.1[医药卫生—生物医学工程]