逆转录病毒靶向介导HDV核酶体外抑制HBV表达  

Inhibition of HBV Expression In Vitro by Targeting Retroviral Vector-mediated HDV Ribozyme

作　　者：戚鹏[1] 韩金祥[1] 鲁艳芹[1] 王传玺[1] 

机构地区：[1]卫生部国家重点实验室

出　　处：《中国生物制品学杂志》2007年第4期233-236,共4页Chinese Journal of Biologicals

基　　金：国家自然科学基金资助项目(编号30371328);山东省自然科学基金资助项目(Q99015)

摘　　要：目的包装携带HDV核酶的肝细胞靶向性逆转录病毒载体,并检测体外HDV核酶对乙型肝炎病毒表达的影响。方法采用脂质体法,分别包装出携带针对HBV不同基因组区域的HDV核酶的肝细胞靶向性重组逆转录病毒,感染HepG2215细胞,ELISA及荧光定量PCR检测HDV核酶对乙型肝炎病毒表达的抑制作用。结果pMSCV-U6-PreS2-Rz和pM-SCV-U6-C-Rz对乙型肝炎病毒表面抗原抑制率明显高于其他HDV核酶和对照组,分别达80%和85%,且高于脂质体转染介导的同种核酶对乙型肝炎病毒表面抗原表达的抑制率。结论靶向性重组逆转录病毒能够携带HDV核酶进入HepG2215细胞,并且对乙型肝炎病毒的表达有较高的抑制作用,为抗病毒治疗的研究奠定了基础。Objective To package the hepatocyte-targentign recombinant retroviral vector carrying HDV ribozyme gene and study the influence of HDV ribozyme on expression of HBV in vitro. Methods The hepatocyte-targeting recombinant retroviral vectors carrying the genes encoding ribozymes specific for different genomes of HBV were packaged by liposome method and used for the infection of HepG2215 cells, and the expression of HBV was determined by ELISA and fluorescent real-time quantitative PCR. Results The inhibition rates of HBsAg by pMSCV-U6-PreS2-Rz and pMSCV-U6-C-Rz were 80% and 85% respectively,which were significantly higher than those by the recombinant retroviral vectors carrying other ribozyme genes as well as that by empty vector plasmid pMSCV- U6, and significantly higher than those by the ribozymes of the same kinds（68% and 60% respectively） in mediation of liposome. Conclusion HDV ribozymes were expressed in HepG2215 cells in mediation of recombinant retroviral vector and inhibited the expression of HBsAg significantly, which laid a foundation of antiviral therapy of lib in vivo.

关 键 词：靶向性逆转录病毒载体 HDV核酶 乙型肝炎病毒 

分 类 号：R373.9[医药卫生—病原生物学]