太极拳运动对老年人骨骼肌全基因组表达的影响  

Global gene expression in human skeletal muscle of the elderly person influenced by Tai Chi Chuan training

作　　者：柯杰兵[1] 马文丽[1] 钟梅[2] 姜立[1] 林建棣[3] 郑文岭[1] 

机构地区：[1]南方医科大学基因工程研究所,广州510515 [2]南方医科大学附属南方医院 [3]中国人民解放军军事体育进修学院军事体育理论教研室

出　　处：《中国康复医学杂志》2007年第4期306-309,322,共5页Chinese Journal of Rehabilitation Medicine

摘　　要：目的:研究太极拳运动对骨骼肌全基因组表达的影响及太极拳促进健康的分子机制。方法:6名健康的老年人(65.5±8.9岁)参加了为期12周的太极拳训练。在训练前后分别对实验对象进行肌活验,提取总RNA,经处理后与Affymetrix U133A基因芯片进行杂交,分析数据。结果:太极拳运动使老年人骨骼肌全基因组表达发生明显改变,筛选出725条表达有差异的基因。本文对表达差异最显著的20条差异表达基因进行研究(3条基因表达上调,17条基因表达下调)。根据基因功能分类对比,差异表达基因分别归属8种细胞组分和生物过程,经KEGG搜索找到4条基因的代谢途径。结论:太极拳运动有助保持神经系统的灵敏性,提高反应能力,有助于抗衰老和减肥等,而不利于骨骼肌蛋白质的合成。Objective： In order to evaluate the effect of Tai Chi Chuan （TCC; shadow boxing） on global gene expression in human skeletal muscle and study the molecular mechanism in improving fitness by TCC. Method： Six health elderly men （65.5±8.9yr of age） participated TCC training for 12 weeks. Needle biopsies were obtained from the skeletal muscle before and after the training course. RNA extracted from the samples was hybridized to Affymetrix UI33A platform and analyzed the gene expression data. Result： TCC training improved the overall physical fitness. Number of genes that passed filtering criteria were 725. The most differently expressed genes were selected in which there were 3 genes upregulated and 17 genes downregulated. According to GO Annotations, the differential genes were classified into 8 GO categories concerning Cellular Component Biological Process, KEGG Searching found 4 genes metabolism pathway. Conclusion： Systematic TCC training can upregulate exprssion of genes concerning energy metabolism and downregulate expression of genes concerning protein metabolism. TCC is good at protect human nerves sphingolipid integrity, which is perhaps the main reason for anti-aging, but it isn＇t beneficial for protein synthesis.

关 键 词：BRB阵列工具 KEGG搜索 基因表达 数据挖掘 太极拳 

分 类 号：R455[医药卫生—运动医学] R49[医药卫生—治疗学]