反相高效液相色谱法分析禾谷丝核菌菌丝中麦角甾醇和羊毛甾醇的含量  

Determination of Ergosterol and Lanosterol in Mycelium of Rhizoctonia Cerealis by Reversed Phase High Performance Liquid Chromatography

作　　者：夏晓明[1] 王金花[2] 王开运[1] 刘振龙[1] 段海明[1] 

机构地区：[1]山东农业大学植物保护学院 [2]上海交通大学环境科学与工程学院,上海200240

出　　处：《分析化学》2007年第2期255-258,共4页Chinese Journal of Analytical Chemistry

基　　金：国家"十五"科技攻关项目(No.2002BA516A12)资助

摘　　要：采用反相高效液相色谱法,建立了检测禾谷丝核菌中麦角甾醇和羊毛甾醇含量的方法。样品经过破碎、离心、皂化后定量测定。采用DiamonsilTMC18色谱柱(250 mm×4.6 mm i.d,5μm i.d.),流动相为100%甲醇,流速为1 mL/min,紫外检测波长为210 nm,保留时间定性,外标法定量。麦角甾醇和羊毛甾醇的保留时间分别为21 min和27min。麦角甾醇在0.125-12.5 mg/L、羊毛甾醇在0.1-10.0 mg/L范围内,两者峰面积与浓度均呈线性关系,相关系数分别为0.9993和0.9983。添加回收率实验表明,2种甾醇的平均添加回收率为91.8%-99.7%;相对标准偏差为2.8%-7.8%;麦角甾醇和羊毛甾醇的最小检出量分别为1.0和0.8 ng,最低检出浓度分别为0.05和0.04 mg/L。本方法准确、简便、重现性好。A determination method of ergosterol and lanosterol in mycelium of Rhizoctonia cerealis by reversed phase high performance liquid chromatography （RHPLC） was established. The sample was analyzed after broke up, centrifugating and saponifiing. The chromatographic column Diamonsil^TM C18 （250 mm ×4.6 mm i. d. , 5 μm i. d） was used with 100% methanol as the mobile phase. The flow rate was 1 mL/min and the detected wavelength was set at 210 nm. The analytes were qualitated by retention time and quantitated by peak area using external standard method. The results show that the retention time of ergosterol and lanosterol is 21 and 27 rain respectively. There are excellent linearity between peakarea and concentration of analytes in the concentration range of 0. 125 - 12.5 mg/L for ergosterol and 0.1 - 10.0 mg/L for lanosterol, the correlation coefficient are 0. 9993 and 0. 9983 respectively. The average recoveries are 91.8% - 99.7%, the relative standard deviations are 2.8% -7.8%. The detection limit of quantity is 1.0 ng and 0.8 ng respectively, the detection limit of concentration is 0.05 mg/L and 0.04 mg/L respectively. This method is simple, accurate and precise.

关 键 词：反相高效液相色谱 禾谷丝核菌 麦角甾醇 羊毛甾醇 

分 类 号：S481.4[农业科学—农药学]