荷瘤小鼠扶正抑瘤颗粒含药血清对H_(22)细胞的凋亡、自由基含量和线粒体膜电位的影响  

Effect of Fuzheng Yiliu Granule Contained-serum from Tumor Bearing Mice on Apoptotic Rate, Free Radicals Content and Mitochondrial Membrane Potential of Hepatoma Cell Lines H22 in vitro

作　　者：王学习[1] 赵健雄[1] 陈茹[1] 白德成[2] 

机构地区：[1]兰州大学中西医结合研究所,兰州730000 [2]兰州大学医学实验中心

出　　处：《中国中西医结合杂志》2007年第4期343-346,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：教育部科学技术研究重点项目(No01128)

摘　　要：目的观察荷瘤小鼠扶正抑瘤颗粒(FYG)含药血清对H22细胞的凋亡、自由基含量和线粒体膜电位的影响。方法应用流式细胞仪检测FYG含药血清对H22细胞凋亡情况的影响;应用激光共聚焦显微镜检测H22细胞DNA RNA、自由基含量和线粒体膜电位的变化。结果FYG含药血清可引起H22细胞的凋亡,细胞DNA RNA和线粒体膜电位降低,自由基含量升高。结论FYK含药血清引起H22细胞的凋亡可能与其影响H22细胞自由基含量和线粒体膜电位变化有关。Objective To observe the effect of traditional Chinese herbs Fuzheng Yiliu Granule （FYG）- contained serum from tumor bearing mice on apoptotic rate, free radicals content and mitochondrial membrane potential of hepatoma cell lines H22 in vitro. Methods The effect of FYG drug-serum on apoptosis of hepatoma cell line H22 was determined using flow cytometry. The changes of DNA RNA, free radicals and mitochondrial membrane potential （△φbm） in H22 cell were detected through laser scanning confocal microscope. Results FYG-contained serum can induce the apoptosis of H22 cell, enhance the free radicals content, and reduce the content of DNA RNA and Aφm of 1-122 cell in vitro. Conclusion The apoptosis of hepatoma cell line H22 induced by FYG is probably correlated to the change of free radicals content and Aφm.

关 键 词：扶正抑瘤颗粒 中药血清 自由基 线粒体膜电位 

分 类 号：R285.5[医药卫生—中药学]