大花蕙兰遗传多样性及亲缘关系的AFLP分析  

Genetic Diversity and Relationship of Hybrid Cymbidium Based on AFLP Marker

作　　者：朱根发[1] 李冬梅[1] 郭振飞[2] 

机构地区：[1]广东省农业科学院花卉研究所,广州510640 [2]华南农业大学生命科学学院,广州510642

出　　处：《园艺学报》2007年第2期417-424,共8页Acta Horticulturae Sinica

基　　金：广东省科技攻关重大专项(2003A2010401;2006A2010401);广州市农业局招标项目(GK0302105)

摘　　要：对来源于日本、韩国和美国的42个大花蕙兰品种和两个国产兰属原生种进行了遗传多样性和亲缘关系的AFLP分析,9对多态性引物在50～500bp内共扩增出1597条带,其中多态性带1565条,多态性比率98.0%。单引物对扩增的带数156～193条,平均每对引物扩增带数177条。42个品种具特征带或缺失带。大花蕙兰品种间的遗传多样性丰富,品种间的相似系数0.3399～0.8223,平均相似系数0.5783。UPGMA聚类结果将供试品种分为4大类,与根据花枝类型或花径大小、花色等形态指标分类的结果相吻合,同一产地来源甚至同一育种公司选育出的品种能基本上聚类在一起,反映出了品种间的亲缘关系。The genetic diversity and relationship of 42 cuhivars of hybrid Cymbidium introduced from Japan, Korea and USA, and 2 wild species from China （ C. aloifolium and C. floribundum） were evaluated using AFLP molecular markers in this study. Among the 1 597 AFLP hands obtained from nine selective primer pairs, 1 565 （98.0%） were polymorphic. The average number of DNA bands per primer pair was 177, with a range of 156 - 193. All the cuhivars have their characteristic bands and can he identified based on the AFLP markers. Genetic similarity based on dice coefficients among the cultivars ranged from 0. 3399 to 0. 8223, with an average of 0. 5783. The cuhivars could be divided into four groups based on the UPGMA dendrogram, and this division is in consistence with the morphological classification based on the type of flower inflorescence, flower size or flower color. Cuhivars originated from the same country or the same breeding company could be clustered together in the AFLP dendrogram and this represents the genetic relationship of the cultivars.

关 键 词：兰科 大花蕙兰 AFLP 遗传多样性 亲缘关系 

分 类 号：S682.31[农业科学—观赏园艺]