蝴蝶兰叶片离体培养胚状体的发生及组织学观察  

Efficient Somatic Embryogenesis from Leaf Explants of Phalaenopsis in Vitro Culture and Histological Observations

作　　者：崔广荣[1] 侯喜林[1] 张子学[2] 张从宇[2] 胡能兵[2] 刘跃成[2] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]安徽科技学院植物科学系,安徽凤阳233100

出　　处：《园艺学报》2007年第2期431-436,共6页Acta Horticulturae Sinica

基　　金：安徽省自然科学基金项目(070411010);安徽省科技厅重点项目(04023069)

摘　　要：以蝴蝶兰试管苗幼叶为外植体,以1/2MS为基本培养基,研究了不同浓度6-苄基腺嘌呤(6-BA)、腺嘌呤硫酸盐(Ad)、萘乙酸(NAA)及其组合对胚状体发生的影响,同时也探讨了蔗糖浓度及椰汁添加量对胚状体形成的影响。结果表明:细胞分裂素6-BA对胚状体的诱导效果较Ad好,NAA与二者配合使用可提高胚状体的发生率,其最佳浓度组合为6-BA4.0mg/L+Ad2.0～4.0mg/L+NAA1.0～2.0mg/L。蔗糖20～40g/L+椰汁200mL/L有利于胚状体的形成。组织学观察表明,胚状体起源于叶片气孔附近的上表皮细胞或上表皮下方的叶肉组织细胞,为单细胞起源,其发育过程与一般植物胚状体发生发育特征相似,最终发育成为类原球茎。The leaves of Phalaenopsis were used as explants and cultured in the 1/2MS basic medium with different kinds of plant growth regulators for somatic embryos induction. The effects of different concentrations of three kinds of plant growth regulators of 6-BA, Ad, NAA and their combinations on somatic embryogenesis were studied in in vitro culture. Meanwhile, the influences of the concentrations of sucrose and coconut water on somatic embryogenesis were studied, too. The results showed that the effects of cytokinins 6- benzyladenine （6-BA） was better than adenine sulphate （Ad） in embryoid induction. The auxin A-naphthalene acetic acid （NAA） combined with given concentrations of 6-BA and Ad could effectively raise the frequency of somatic embryogenesis. The optimum plant was 6-BA 4. 0 mg/L ＋ Ad 2. 0 - 4. 0 mg/L ＋ NAA 1. growth regulator combination for somatic embryogenesis 0 -2. 0 mg/L. 20 -40 g/L sucrose and 200 mL/L coconut water added in the medium was of benefit to somatic embryogenesis. The results of histological observations indicated that somatic embryos originated from upper epidermis and mesophyll single cell nearby stoma. The course of somatic embryos development was similar to other plants. Finally, the somatic embryos became protocorm-like body.

关 键 词：蝴蝶兰 叶片 胚状体 组织培养 组织学观察 原球茎 

分 类 号：S682.31[农业科学—观赏园艺]