苹果花药培养植株倍性及其纯合基因型的鉴定  

Analysis of Ploidy and Homozygous Genotype of Apple Plants Obtained by Anther Culture

作　　者：张利义[1] 杨振英[1] 丛佩华[1] 张开春[2] 刘建利[3] 薛光荣[1] 

机构地区：[1]中国农业科学院果树研究所,辽宁兴城125100 [2]北京市农林科学院林业果树研究所,北京100093 [3]西北第二民族学院生命科学系,银川750021

出　　处：《园艺学报》2007年第2期481-484,共4页Acta Horticulturae Sinica

基　　金：国家‘863’资助项目(2001AA241144)

摘　　要：用倍性分析仪对3株苹果花药培养植株进行了倍性鉴定,并用AS-PCR分子标记分析其是否为纯合体。流式细胞法测定其倍性分别为二倍体、三倍体和四倍体;AS-PCR分析证明它们为纯合基因型。可认为花药培养植株的多倍化现象可能是在组织培养条件下,由起源于单倍体的细胞发生自然加倍所形成的结果。In this paper, the ploidy and homozygous genotype of three apple plants obtained by anther culture were analyzed by using methods of the flow cytometric analysis and AS-PCR marker, in order to confirm their haploid origin. The results have proved that they all have a true homozygous status by AS-PCR analysis although they are now diploid, triploid and tetraploid respectively by Ploidy Analyser, which is possibly regarded as a result of spontaneous polyploidisation from haploid cell during tissue culture.

关 键 词：苹果 花药培养 AS—PCR 倍性 纯合基因型 

分 类 号：S661.1[农业科学—果树学]