阿托伐他汀对大鼠局灶性脑缺血/再灌注损伤的保护作用  

The protective effect of atorvastatin(Lipitor) against focal cerebral ischemia-reperfusion injury in rats

作　　者：王德超[1] 李会敏[1] 王彬生[2] 申文增[3] 陈立英[1] 

机构地区：[1]河北大学附属医院神经内科,河北保定071000 [2]河北大学附属医院核医学科,河北保定071000 [3]河北大学医学部中心实验室,河北保定071000

出　　处：《广东医学》2007年第5期687-689,共3页Guangdong Medical Journal

基　　金：河北省科学技术与发展课题(编号:0527611024)

摘　　要：目的探讨阿托伐他汀对大鼠局灶性脑缺血/再灌注损伤的保护作用。方法采用Longa等方法制作大鼠大脑中动脉局灶性脑缺血/再灌注损伤模型。60只雄性SD大鼠随机分为3组,假手术组(SH组),脑缺血/再灌注组(ISC组)和阿托伐他汀预处理组(LipPC组),在缺血/再灌注前按6 mg/(kg.d)给阿托伐他汀14 d。各组又按再灌注后6 h和24 h分成两个亚组,每个亚组10只。各组在相应时间点进行神经功能评分,测定脑梗死体积、血清神经元稀醇化酶(NSE)含量、脑组织TNF-α和IL-1β的含量。结果LipPC组各时间点梗死体积比ISC组显著缩小(P<0.05),神经功能评分明显改善(P<0.05),血清NSE、脑组织TNF-α和IL-1β生成减少(P<0.05或P<0.01)。结论缺血前给予阿托伐他汀可减轻缺血/再灌注后的炎症级联反应,对大鼠局灶性脑缺血/再灌注损伤有一定的保护作用。Objective To determine whether Atorvastatin（Lipitor） has protective effect against focal cerebral ischemia - reperfusion injury in rats. Methods A rat model of middle cerebral artery ischemia/reperfusion injury was established accarding to the method reported by Longa sixty male SD rats were randomly divided into three groups： sham group（ SH group）, cerebral ischemia/reperfusion group （ ISC group） and Lipitor preconditioning group （ LipPC group）, which received Liptor（6 mg/（ kg·d） ）treatment before ischemia/reperfusion. Each group was sub -divide into two sub - groups at 6 h and 24 h after reperfusion. Each subgroup consisted of 10 rats. At 6 h and 24 h after reperfusion, the animals were observed for neurological deficits and the volume of cerebral infarction was estimated, The level of NSE in serum and the contents of tumor necrosis factor - alpha（ TNF - α） and interleukin - 1 beta（ IL - 1β） in brain tissue were measured. Results Compared with ISC group, the behavior score and infarct volume in LipPC group were significantly reduced remaikably（P 〈0. 05） ;the level of NSE in serum and the contents of TNF - α and IL - 1β in LipPC group were significantly decreased（ P 〈 0.05 or P 〈 0. 01 ）. No differences were found in these parameters between LipPC group and SH group（ P 〉 0. 05 ）. Conclusion Pretreatment of Lipitor before ischemia can inhibit inflammation in duced by ischemia/ reperfusion injury and is protective against local cerebral ischemia/repefusion injury.

关 键 词：阿托伐他汀 缺血/再灌注 神经元稀醇化酶 白细胞介素-1Β 肿瘤坏死因子-α 大鼠 

分 类 号：R541[医药卫生—心血管疾病]