耳压及防石胶囊预防胆囊结石的实验研究  

Experimental Studies on Prevention of Gallstone with Fangshi Capsule and Auricular-plaster Therapy

作　　者：杨卓欣[1] 李健[1] 张永斌 黄明河[1] 张竞超[1] 郭绍举[1] 

机构地区：[1]广州中医药大学附属深圳市中医院,深圳518033 [2]广州中医药大学实验动物中心,广州510405

出　　处：《广州中医药大学学报》2007年第3期211-214,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省中医药管理局科研课题(编号:401052);深圳市医学重点专科及科技计划项目(编号:200204236)

摘　　要：【目的】观察耳压及防石胶囊预防胆囊胆固醇和胆红素结石的作用。【方法】①预防胆固醇结石实验:将75只豚鼠随机分成5组,空白组每天只喂普通饲料,模型组每天喂胆固醇结石诱石饲料,熊去氧胆酸(UDCA)组喂胆固醇结石诱石饲料加UDCA(剂量为50 mg/kg),防石组喂胆固醇结石诱石饲料加防石胶囊(剂量为1 g/kg),耳压组喂胆固醇结石诱石饲料并每天按压耳穴中胆俞、胆囊穴及日月等穴;②预防胆红素结石实验:将64只豚鼠随机分成4组,空白组每天只喂普通饲料,模型组每天喂胆红素结石诱石饲料,防石组喂胆红素诱石饲料加防石胶囊(剂量为1 g/kg),耳压组喂胆红素诱石饲料并每天按压耳穴(穴位同前)。【结果】2个月后检测结果显示:预防胆固醇结石实验中模型组胆囊结石发生率及胆汁中钙离子浓度显著性升高,防石胶囊及耳压法可显著性降低结石发生率及钙离子浓度;预防胆红素结石实验中模型组胆囊结石发生率及胆汁中胆红素含量显著性升高,防石胶囊及耳压法均可显著性降低结石发生率及胆红素含量(均P<0.05或P<0.01)。【结论】耳压及防石胶囊能预防胆囊胆固醇和胆红素结石的形成,其机制可能与它们能分别降低胆汁中钙离子浓度及降低胆红素含量有关。[ Objective] To observe the preventive effect of Fangshi Capsule （FC, mainly composed of Herba Lysimachiae, Rhizoma Curcumae Longae, Radix Clematidis, Radix Paeoniae Rubra, Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Radix Glycyrrhizac, Rhizoma Curcumae, etc. ） and auricular-plaster （AP） therapy on guinea pig cholesterol gallstone and bilimbin gallstone. [ Methods ] （1） Experiment of prevention of cholesterol gallstone： Seventy-five guinea pigs were randomized into 5 groups of A, B, D and E. Except group A was fed with routine forage, the other four groups were fed with cholesterol-gallstone-induced forage. Meanwhile, group C received ursodesoxycholic acid （UDCA） in the dosage of 50 mg,/kg, group D was treated with FC in the dosage of 1 g/kg, and group El with auricular-plaster （AP） therapy on the acupoints of Danshu, Dannang and Riyue additionally. （2） Experiment of prevention of bilimbin stone： Sixty-four guinea pigs were randomized into 4 groups of A, B, D and E. Except group A was fed with routine forage, the other three groups were fed with bilimbin-stone-induced forage. Meanwhile, groups D and E received the same additional treatment as that in experiment （1）. [Results] After 2 months, gallstone incidence and Ca^2 ＋ concentration were increased in the cholesterol-gallstone model group, and FC and AP therapy decreased gallstone incidence and Ca^2 ＋ concentration. Gallstone incidence and bilirubin content were also increased in the bilimbin-gallstone model group, and were decreased in groups D and E （P 〈 0.05 or P 〈 0.01）. [Conclusion ] AP therapy and FC can prevent the formation of cholesterol-gallstone and bilirubin-gallstone, their mechanism may be related to the decrease of Ca^2 ＋ concentration and bilimbin content in the bile respectively.

关 键 词：胆结石/中药疗法 胆结石/穴位疗法 穴 胆俞 穴 胆囊 穴 日月 疾病模型 动物 豚鼠 

分 类 号：R245.9[医药卫生—针灸推拿学]