大黄治疗大鼠系膜增殖性肾炎的实验研究  

EXPERIMENTAL TREATMENT OF RHUBARB ON MESANGIO PROLIFERATIVE GLOMERULONEPHRITIS IN RATS

作　　者：魏建冬[1,2] 黎磊石[1,2] 姚建 

机构地区：[1]南京市鼓楼医院肾内科 [2]南京军区总医院肾脏病研究所

出　　处：《中华内科杂志》1997年第2期87-89,共3页Chinese Journal of Internal Medicine

摘　　要：为了解中药大黄对系膜增殖性肾炎的治疗效果，用计算机图像分析及ＥＬＩＳＡ法观察了大黄对抗Ｔｈｙ－１肾炎大鼠系膜区细胞外基质的影响，并检测了肾小球培养上清中的白细胞介素－１（ＩＬ－１）活性。结果表明，大黄不仅减少了肾炎大鼠基质的堆积，还能降低肾小球培养上清中的纤维连接蛋白（ＦＮ）浓度和ＩＬ－１活性。肾小球ＦＮ浓度在未治疗组非常显著地高于对照组与大黄组（Ｐ＜０．０１），其中大黄组又显著低于对照组（Ｐ＜０．０５）。肾小球ＩＬ－１活性未治疗组显著高于大黄组，而大黄组又显著高于正常对照组（Ｐ＜０．０５）。大黄能改善系膜增殖性肾炎大鼠系膜区基质的堆积，其作用可能与其抑制肾小球分泌ＩＬ－１有关。Accumulation of extracellular matrix is a prominent feature of progressive glomerulonephritis (GN). Previously, we have shown that Emodin, an important component of Rhubarb, inhibits the fibronectin (FN) production of cultured mesangial cells. We now provide evidence of the same effect of Rhubarb in immune complex GN induced in rats by injection of anti thymocyte serum (ATS). Rhubarb ameliorated mesangial matrix expansion on day 7 after ATS administration. Decreased FN level and IL 1 activity were found in the supernatant of cultured glomeruli from Rhubarb treated animal at the 7th day. Glomerular tissue FN fluorescence staining was also much weaker than that of the untreated rats. These studies demonstrated that Rhubarb treated anti Thy 1 animal model should develop significantly less matrix expansion. Rhubarb also inhibited synthesis and secretion of fibronectin, an important component of mesangial extracellular matrix. Decreased IL 1 activity might be involved in the therapeutic effect of Rhubarb on mesangioproliferative glomerulonephritis.

关 键 词：大黄 肾小球肾炎 膜增生性 中医药疗法 

分 类 号：R277.523[医药卫生—中医学]