顺磁共振和傅里叶变换红外光谱法研究高温对荒漠苔藓质膜结构影响  

Studies of Plasma Membrane Structures Responding to Heat Shock in Desert Moss by Electron Paramagnetic Resonance and Fourier Transform Infrared

作　　者：许书军[1] 王艳[1] 陈颖雯[1] 蔡伟民[1] 陈蔚红[2] 

机构地区：[1]上海交通大学环境科学与工程学院,上海200240 [2]中国科学院上海有机化学研究所,上海200032

出　　处：《分析化学》2007年第3期350-354,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(No.30371002)

摘　　要：运用顺磁共振波谱和原位傅立叶变换显微红外光谱研究了高温对荒漠植物刺叶墙藓不同叶龄水合组织质膜透性和膜蛋白二级结构的影响。自旋标记法研究质膜透性结果表明,处理前野生叶质膜透性普遍高于室内培养获得的原丝体和次生叶,处理后次生叶膜透性变化最大,比处理前增加4倍,其次为原丝体。质膜透性随叶龄增加,变化幅度逐渐降低。红外光谱的二阶求导、傅立叶自解卷积及拟合分峰等结果显示,各叶龄间蛋白质二级结构含量差异较大,表明各龄组织蛋白成分不完全相同;高温处理后次生叶和原丝体α螺旋含量分别比对照增加40%和16%;其它叶龄组织二级结构含量变化范围小,表明热胁迫下老龄组织蛋白质二级结构稳定。膜透性和膜蛋白二级结构分析证明,膜透性和蛋白质稳定性呈正相关,指示蛋白质组成和含量不同是造成各龄组织不同耐热性的主要原因之一。The effect of thermostress shock on the membrane permeability and protein secondary structure of the desert moss Tortula desertorum was investigated by electron paramagnetic resonance and in situ Fourier transform infrared microspectroscopy. The results of spin label showed that plasma membrane permeability was changed significantly among different aging tissues and increased by aging in control level. However, after treated at 45 ℃ for 1 h, membrane permeability was promoted by contraries to aging. The changes in secondary leaves and protonema were most pronounced, increased 4 doubles after treatment. The protein secondary structure of T. desertorum was affected by thermostress through analyzing the spectra of FFIR using second-derivative, Fourier self-deconvolution and curve-fitting. The components of protein secondary structure were obviously different among various aging leaves, indicating that types of proteins changed in leaves. The content of α-helix was increased 40% and 16% in secondary leaves and protonema respectively after thermostress than that of initial. Aging leaves were little changes in protein secondary structure, it showed there was no dramatically damage to them. The results of membrane permeability and protein secondary structure proved the proteins stability were negative correlation with changes of permeability, indicating types and content of proteins might be one of the most important reasons causing aging leaf difference in response to high temperatures.

关 键 词：质膜结构 耐热性 自旋标记 原位红外 刺叶墙藓 

分 类 号：O657.33[理学—分析化学]