铜纳米微粒与维生素B_1相互作用的吸收光谱和共振瑞利散射光谱研究  

Absorption and Resonance Rayleigh Scattering Spectra of the Interaction for Copper Nanoparticles with Vitamin B_1

作　　者：王齐[1] 刘忠芳[1] 孔玲[1] 刘绍璞[1] 

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《分析化学》2007年第3期365-369,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(No.20475045)

摘　　要：在过量碘离子（I-）存在下,用硼氢化钠还原硫酸铜溶液,得到Cu纳米微粒（Cun）。Cu纳米微粒因表面吸附I-而带负电荷。在弱碱性介质中,它能与正电荷的维生素B1（VB1）反应形成结合产物,此时不仅引起吸收光谱的变化,而且导致共振瑞利散射的显著增强,其最大RRS波长位于369nm处。VB1浓度在0.02-0.40mg/L范围内与散射强度（ΔI）强度成正比。方法具有较高灵敏度,对VB1检出限为6.4μg/L;可用于复合维生素中维生素B1含量的测定。据此建立了一种以Cu纳米微粒探针用RRS技术灵敏、简便、快捷测定VB1的新方法。A sensitive, simple and fast method for the determination of Vitamin B1 with nanoparticles as probe by resonance Reyleigh scattering （RRS） technique has been developed. In the presence of excess I^- , copper nanoparticles were prepared by using the reduction reaction of NaBH4 and copper sulphate. In the solution, I^- anions self-assemble on the surface of copper nanoparticles and become large anionic complexes [ CunIm ]^x- It can react further with Vitamin B1 cations to form binging products, resulting in the change of the absorption spectrum and great enhancement of RRS intensity. The maximum scatting wavelength is 369 nm. There was a linear relationship between the RRS intensity（AI） and the Vitamin B1 concentration in the range of 0.02 -0.40 mg/L. The detection limit for Vitamin B1 was 6.4 μg/L.

关 键 词：Cu纳米微粒 维生素B1 共振瑞利散射 吸收光谱 

分 类 号：O657.3[理学—分析化学]