溶剂浮选法分离富集淫羊霍提取液中淫羊霍苷  

Separation and Enrichment of Icariin in Extract of Epimedium by Solvent Floatation

作　　者：王玉堂[1] 张佳秋[1] 于永[1] 李娟[1] 丁兰[1] 张寒琦[1] 

机构地区：[1]吉林大学化学学院,长春130012

出　　处：《分析化学》2007年第3期409-412,共4页Chinese Journal of Analytical Chemistry

基　　金：吉林省科技发展计划项目(No.20050560)

摘　　要：应用溶剂浮选法对淫羊霍提取液中的淫羊霍苷进行分离富集,并用高效液相色谱法测定淫羊霍苷的含量。考察了浮选溶剂的用量、氮气流速、浮选液pH值、浮选时间和电解质NaCl浓度对浮选效率的影响,并与泡沫浮选法以及溶剂萃取法进行了比较。结果表明溶剂浮选法分离富集的效果最好,泡沫浮选法次之,溶剂萃取法最差,富集倍数分别为6.4、5.9和1.4倍,收率分别为64.4%、58.8%和28.4%。A solvent floatation technique was applied for the separation and enrichment of icariin in extract from Epimedium and high performance liquid chromatography （HPLC） was applied to determining icariin. The effects of volume of solvent, pH of the solution, the flow rate of nitrogen gas, floatation time and the NaCl concentration on the floatation efficiency were investigated. The optimal conditions were selected. Solvent floatation was compared with foam floatation and solvent extraction. The experimental results showed that the highest efficiency of separation and enrichment were obtained by solvent floatation. When solvent floatation, foam floatation and solvent extraction were applied, the enrichment factors obtained are 6.4, 5.9 and 1.4, and the yields obtained are 64.4%, 58.8% and 28.4%, respectively.

关 键 词：溶剂浮选法 淫羊霍 淫羊霍苷 高效液相色谱法 

分 类 号：R286[医药卫生—中药学]