选择性环氧化酶2抑制剂对梗阻性肾病大鼠肾组织中核因子кB和转化生长因子β_1的调节  

Effect of celecoxib on activation of nuclear factor-kappa B and transforming growth factor-β_1 in obstructive nephropathy

作　　者：何平[1] 杨旭[1] 李德天[1] 

机构地区：[1]中国医科大学附属盛京医院肾内科,辽宁沈阳110004

出　　处：《中国医科大学学报》2007年第2期143-144,共2页Journal of China Medical University

摘　　要：目的:探讨塞来昔布与吲哚美辛比较对单侧输尿管梗阻(UUO)大鼠模型肾脏的保护作用及可能机制。方法:将塞来昔布和吲哚美辛应用于UUO大鼠,4周后测定血清肌酐及尿素氮水平,放射免疫法测定大鼠尿液血栓烷素B(2TXB2)浓度,免疫组化法检测肾脏核因子кB(NF-кB)和转化生长因子β(1TGF-β1)的表达。结果:塞来昔布及吲哚美辛均降低尿TXB2浓度,塞来昔布组更为显著;与对照组相比,其余3组NF-кB及TGF-β1的蛋白表达水平均显著上升,肾间质纤维化严重;塞来昔布治疗后,上述蛋白表达水平显著下调,肾间质纤维化程度明显减轻。结论:塞来昔布能减轻UUO大鼠肾间质纤维化,下调NF-кB的表达,减少肾间质单核巨噬细胞浸润可能是其机制之一。Objective： To investigate the protective effects of celecoxib and indomethacin on renal interstitial fibrosis following unilateral ureteral obstruction （UUO） in rat kidney and the mechanism, Methods： Twenty-four Wistar rats were randomly and equally divided into UUO group,celecoxib group,indomethacin group,and sham operation group. The concentration of thromboxane-B2 （TXB2） in the urine was detected by radioimmunoassay after 4 weeks, and the expressions of nuclear factor-kappa B （NF-KB） and transforming growth factor-β1 （TGF-β1） were determined by immunohistochemical method. Results： Both celecoxib and indomethacin,especially celecoxib,reduced the concentration of TXB2. Compared with sham operation group,the levels of NF-κB and TGF-β1 were significantly increased and the renal interstifial fibrosis was more severe in other 3 groups. Celecoxib signifcanfly suppressed the expression of NF-xB and TGF-β1 and improved renal interstitial fibrosis. Conclusion： Celecoxib could ameliorate the renal interstitial fibrosis in UUO mrs,and the possible mechanism may include down-regulating the expression of NF-κB and reducing the renal macrophage infiltration.

关 键 词：肾间质纤维化 塞来昔布 梗阻性.肾病 核因子ΚB 转化生长因子β1 

分 类 号：R693[医药卫生—泌尿科学]