多西紫杉醇对MCF-7人乳腺癌细胞延迟外向钾电流的作用  

Effect of Docetaxel on delayed rectifier potassium current in human breast cancer cell line MCF-7

作　　者：孙涛[1] 姜大庆[2] 宋志国[3] 李金鸣[3] 

机构地区：[1]辽宁省肿瘤医院内科,辽宁沈阳110042 [2]辽宁省肿瘤医院乳腺科 [3]中国医科大学药学院药理学教研室

出　　处：《中国医科大学学报》2007年第2期199-200,共2页Journal of China Medical University

基　　金：辽宁省博士启动基金资助项目(20051025)

摘　　要：目的:探讨多西紫杉醇对MCF-7人乳腺癌细胞延迟外向钾电流(IK)的作用。方法:采用全细胞膜片钳记录方式,设保持电位-90mV,刺激电位为-60～+60mV,步阶脉冲10mV,波宽200ms,刺激间隔3s,刺激频率为2Hz。结果:随着多西紫杉醇的浓度增加,MCF-7细胞IK电流电压曲线斜率减少,多西紫杉醇对IK通道具有关闭作用;在同一指令电压下,IK随着多西紫杉醇浓度的增加而减少,具有剂量依赖性,当指令电压为+60mv时,多西紫杉醇浓度从1×10-7mol·L-1增加到1×10-5mol·L-1时,IK从(124.03±8.43)pA/pF,减少到(75.16±9.10)pA/pF,每个浓度的IK与给药前相比,差异显著(P<0.01)。结论:多西紫杉醇对MCF-7人乳腺癌细胞的延迟整流K+通道具有关闭作用,能够抑制乳腺癌细胞的增殖。Objective： To investigate the effect of Docetaxel on delayed rectifier potassium current （Ik） in breast cancer cell line MCF-7. Methods： Whole cell patch clamp technique （-90 mV holding potential,-60 mV to ＋60 mV stimulating potential, ＋10 mV step potential, 200 ms duration,3 s stimulating interval,and 2 Hz stimulating frequency） was used. Results： The slopes of current-voltage curve of Ik decreased with the increase in the concentration of Docetaxel. At ＋60 mV,the current density of Ik decreased from 124.03±8.43 to 75.16±9.10 pA/pF when the concentration of Docetaxel increased from 1×10^-7 to 1 ×10^-5mol/L,and in this process Docetaxel acted in a concentrationdependent manner. The differences in current density between cells untreated with Docetaxel and those treated with different concentrations of Docetaxel were significant （P 〈 0.01 ）. Conclusion： Docetaxel has the closing effect on Ik,and as a result it inhibits the proliferation of breast cancer cells.

关 键 词：乳腺癌 多西紫杉醇 钾离子通道 延迟整流钾电流 

分 类 号：R979.1[医药卫生—药品]