去甲基万古霉素高产菌株的诱变选育  

Breeding on N-demethylvancomycin High-producing Strain

作　　者：闫敬[1] 程冀平[1] 代丽敏[1] 翟妍蕊 张利利[1] 

机构地区：[1]华北制药集团新药研究开发有限责任公司,河北石家庄050015

出　　处：《河北化工》2007年第5期36-37,共2页Hebei Chemical Industry

摘　　要：去甲基万古霉素产生菌东方拟无枝菌酸菌320,经紫外线诱变、激光照射复合处理后分离,在含有去甲基万古霉素的平板上筛选去甲基万古霉素抗性突变株。得到的抗性变株05-8-85,效价比出发株高32.1%,且遗传稳定性良好、发酵周期缩短24h。The N-demethylvancomycin high-producing strain, 05-8-85, with the yield of N-demethylvancomycin was improved 32. i% than parent strain 320, was obtained from N-demethylvancomycin resistant screening plate after mutated by UV （30 W, 30 cm, 90 s） and irradiated by laser （40 mV,15 min）.The characteristic of high-producing was passed down steadily. In addition, the perieod of fermentation was shortened for 24 h.

关 键 词：去甲基万古霉素 东方拟无枝菌酸菌 复合诱变 

分 类 号：R978.1[医药卫生—药品]