悬浮芯片在核酸和蛋白质检测中的应用  

Applications of Suspension Array of Nucleic Acid and Protein Detection

作　　者：罗渊[1] 刘伯华[1] 祝庆余[1] 

机构地区：[1]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071

出　　处：《微生物学杂志》2007年第2期78-82,共5页Journal of Microbiology

摘　　要：悬浮芯片是近年来兴起的一种新型检测技术,不同于固相基因芯片,它整合了高分子化学、分子生物学、免疫学、激光检测、微流体、高速数字信号处理、计算机分析等方面的先进技术,能够对少量样本进行高通量的定性、定量检测。主要综述了悬浮芯片技术的基本原理,并概要介绍了其在核酸和蛋白质检测中的应用。悬浮芯片技术在核酸和蛋白质检测中有着显著的优点,如高通量、操作简便、重复性好、灵敏度高、线性范围宽等,不但可以广泛应用于科学研究领域,而且还将逐渐普及于临床诊断实验室,具有广阔的应用前景。Suspension array is an emerging detection technology. Different from the conventional solid-phase genechip, it integrated numerous advanced technologies such as polymer chemistry, molecular biology, immunology, laser technology, microfluid, and high-speed digital signal processing and computer analysis. Therefore, it could detect qualitatively and quantitatively with only small amount of sample in high flux. The principle of the technology as well as its application in nucleic acid and protein detection are mainly summarized in this paper. As compared with other detection methods it has notable merits, such as high flux, simple operation, good duplication, high sensitivity, broad linear range etc. It could not only apply in scientific research field, hut also could gradually popularize into clinical diagnosis laboratory, therefore, it has broad application prospect.

关 键 词：悬浮芯片 核酸和蛋白质 高通量检测 

分 类 号：Q503[生物学—生物化学]