流体剪切力对大鼠成骨细胞增殖及细胞周期的影响  

Effects of fluid shear stress on the proliferation and cell cycle of rat osteoblasts in vitro

作　　者：吴丹[1] 丁寅[1] 王琪[1] 

机构地区：[1]第四军医大学口腔医学院正畸科,陕西西安710032

出　　处：《临床口腔医学杂志》2007年第4期197-199,共3页Journal of Clinical Stomatology

摘　　要：目的:观察流体剪切力对大鼠成骨细胞增殖及细胞周期的影响。方法:大鼠成骨细胞受强度为13dyn/ecm2的流体剪切力刺激60min,应用四甲基偶氮唑蓝(MTT)法和流式细胞仪检测细胞的增殖能力和细胞周期。结果:流体剪切力作用后,大鼠成骨细胞增殖能力提高,细胞活性增强。加力组S期细胞百分比(14.67±1.18)%,较对照组S期细胞百分比(8.05±1.08)%约增高82.2(%p<0.01)。结论:流体剪切力具有提高大鼠成骨细胞增殖能力以及增强其细胞活性的作用。Objective： To observe the effect of fluid shear stress （FSS） on the cellular proliferation and cell cycle of rat osteoblasts（OBs）. Method： After rat osteoblasts were stimulated by the FSS, MTr assay and flow cytometry were applied to measure the cellular proliferation and cell cycle. Result： 60 min FSS treatment accelerated cell proliferation significontty. 24 hrs after FSS stimulation, the percentage of phase S cells （14.67±1.18） % was increased 82.2% as compared to that of the control group （8.05±1.08）% （p〈0.01）. Conclusion： FSS plays a positive role in promoting the cellular proliferation of the rat osteoblasts.

关 键 词：大鼠成骨细胞 流体剪切力 细胞增殖 细胞周期 

分 类 号：Q813.1[生物学—生物工程]