地黄合剂对多发性硬化的作用及其作用靶点的研究  

Effect of Dihuang on multiple slerosis and its pathway

作　　者：高聪[1] 谢富华[1] 区腾飞[1] 杨宁[1] 高敏[1] 

机构地区：[1]广州医学院第二附属医院神经内科,广州市510260

出　　处：《实用诊断与治疗杂志》2007年第5期323-325,共3页Journal of Practical Diagnosis and Therapy

基　　金：广东省中医药局项目(项目编号100030);广东省科技攻关项目(2000年广东省第十三批重点项目30;文件号粤科计字[2000]261);番禺区科技计划项目(2006-Z-44-1)

摘　　要：目的:观察地黄合剂对多发性硬化患者中枢神经系统中胶质细胞特异性蛋白神经胶质原纤维酸性蛋白和酸性钙结合蛋白表达的影响,探讨地黄合剂在多发性硬化发生发展过程中的作用。方法:用AI,9HPT对多发性硬化患者进行临床评分及分析它们之间的关系,随访3年内经过不同治疗的多发性硬化患者的复发情况,用ELISA法检测多发性硬化患者中枢神经系统中神经胶质原纤维酸性蛋白和酸性钙结合蛋白含量的变化。结果:在多发性硬化组中枢神经系统中神经胶质原纤维酸性蛋白和酸性钙结合蛋白表达明显增强,与正常对照组比较差异有统计学意义,并且与多发性硬化患者的临床评分(AI,9HPT)存在相关性。激素治疗组与未治疗的多发性硬化组比较差异有统计学意义(P<0.05),地黄合剂+激素治疗组与激素治疗组比较差异有统计学意义(P<0.01),同时地黄合剂+激素治疗组对减少多发性硬化的复发次数与单一激素治疗组比较差异有统计学意义(P<0.01)。结论:地黄合剂通过抑制胶质细胞的激活,减少多发性硬化患者中枢神经系统中神经胶质原纤维酸性蛋白和酸性钙结合蛋白的表达,影响多发性硬化的发生和发展及减少多发性硬化患者复发的次数。Objective To observe the effects of Dihuang on the expression of glial fibrillary acidic protein, S100β in CSF from multiple sclerosis patients, and to explore the role of Dihuang in the development processes of multiple sclerosis. Methods AI and 9HPT scales were used to value the clinical symptoms and the relationship was analyzed. The times of recurring were followed up and recorded in multiple sclerosis patients. The expressions ot glial fibrillary acidic protein and S100β in CSF of multiple sclerosis were detected with ELISA method. Results The expressions of glial fibrillary acidic protein and S100β were stronger in control group than in multiple sclerosis group, hormone therapy group and Dihuang add hormone therapy group, especially than Dihuang add hormone therapy group（P〈0.01）. There were correlations between AI and 9HPT clinical scores and the expressions of glial fibrillary acidic protein and S100β in multiple sclerosis patients. There was a statistic difference in decreasing the times of recurring of multiple sclerosis between hormone therapy group and Dihuang add hormone group （P〈0. 01）. Conclusion Dihuang, a Chinese medicine, by inhibiting the functions and activation of glial cells in multiple sclerosis, can alleviate the symptoms of multiple sclerosis patients and decrease the times of recurrence, which suggests that Dihuang may be an ideal supplemental medicine in preventing and treating multiple sclerosis.

关 键 词：多发性硬化 地黄合剂 神经胶质原纤维酸性蛋白 酸性钙结合蛋白(S100β) 

分 类 号：R277.7[医药卫生—中医学]