嗜盐古生菌br基因的遗传分析  

Genetic analysis of the br gene in halophilic archaea isolated from Xinjiang region

作　　者：徐晓红[1] 吴敏[2] 张会斌 刘志虎 

机构地区：[1]浙江大学宁波理工学院,宁波315100 [2]浙江大学生命科学学院,浙江杭州310027 [3]阿尔金山国家自然保护区管理处,库尔勒841000

出　　处：《遗传》2007年第3期376-380,共5页Hereditas（Beijing)

基　　金：国家自然科学基金资助项目(编号:30370029);浙江大学宁波理工学院科研启动基金资助项目(编号:1140257G413)~~

摘　　要：从新疆阿尔金山地区阿乌拉仔盐湖分离纯化到几株极端嗜盐古生菌AJ11,AJ12和AJ13,采用PCR技术分别扩增了其16S rRNA基因(16S rDNA)和编码螺旋C至螺旋G的细菌视紫红质(bacteriorhodopsin,BR)蛋白基因片段,测定了基因的核苷酸序列。基于16S rDNA序列的同源性比较以及系统发育学研究表明,分离到的菌株是Natrinema属中成员,并构成一个独立的微生物种群。随后的遗传分析,包括GC含量、转换与颠换的比率、同义突变率分析,表明br基因间具有较高的遗传分歧程度,并面临着净化选择和偏倚突变压的双重抑制。研究为物种资源及BR蛋白资源的进一步利用打下基础。Some novel members of extremely halophilic Archaea, strain AJ 11, AJ12 and AJ13, were isolated from Aularz Lake located in Altun Mountain National Nature Reserve of Xinjiang Uygur Autonomous Region in China. Partial DNA fragments encoding a bacteriorhodopsin （BR） as well as for 16S rRNA of isolated strains were amplified by PCR and their DNA sequences were determined subsequently. On the basis of homology and phylogenetic analysis about 16S rDNA, it was considered that the isolated strains formed a microbiological population are the members of genus Natrinema. The results of genetic analysis, such as GC content, transition/transversion （Ti/Tv） rate ratios, synonymous substitution rates （Ks）, indicated that the br fragments with high level of genetic divergence are faced with both purifying selection and bias mutation pressure. The study provides the base for using of species and BR proteins resources.

关 键 词：嗜盐古生菌 16S RDNA 系统发育 细菌视紫红质 遗传分析 

分 类 号：Q933[生物学—微生物学]