癌-睾丸抗原SCP-1 mRNA在人鼻咽癌中的表达  

Expression of cancer-testis antigen SCP-1 mRNA in human nasopharyngeal carcinoma

作　　者：以敏[1] 廖红[1] 黄绍明[1] 谢小薰[1] 罗国容[1] 

机构地区：[1]广西医科大学组胚教研室,南宁530021

出　　处：《临床耳鼻咽喉头颈外科杂志》2007年第8期343-345,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：广西科技厅基金(桂科基:05750599);国家自然科学基金资助项目(No:30160024)

摘　　要：目的:从基因水平了解癌-睾丸抗原SCP-1在人鼻咽癌(NPC)的表达情况。方法:用逆转录-聚合酶链反应(RT-PCR)技术,检测28例NPC组织和40例非癌鼻咽部组织SCP-1 mRNA的表达。随机抽取SCP-1表达阳性的PCR产物进行DNA测序并结合临床指标进行统计学处理。结果:SCP-1 mRNA在NPC的表达率为14.3%(4/28),在非癌鼻咽部组织无表达;测序结果表明PCR扩增产物序列与目的基因序列相符;SCP-1 mRNA的表达率与NPC患者的年龄、性别和血清抗EB病毒水平无相关性(P>0.05)。结论:SCP-1 mRNA在NPC组织中有表达,提示SCP-1可能是一种新的NPC肿瘤靶抗原。Objective：To investigate the gene expression of Cancer-Testis Antigen SCP-1 in human nasopha- ryngeal carcinoma. Method：The SCP-1 mRNA were examined in the tumor tissues of 28 nasopharyngeal carcinoma cases and control pharynx nasitis tissues of 40 non-cancer cases by reverse transcriptase polymerase chain reaction. A randomized sample of positive expression of SCP-1 mRNA was extracted for DNA sequencing to examine the reliability of results. The correlation of the expression of SCP-1 mRNA with clinical data was analyzed by statistical method. Result：The expression frequency of SCP-1 mRNA was 14.3% （4/28） in nasopharyngeal carcinoma and zero （0/40） in control pharynx nasitis tissue of non-cancer cases （P〈0.05） ; The DNA sequencing confirmed that the product of reverse transcriptase polymerase chain reaction was truly the target gene; No significant relationship was found between the expression of SCP-1 mRNA and clinical characters, such as age, gender and serum anti-EBV level （P〉0.05）. Conclusion：SCP-1 mRNA is expressed in some nasopharyngeal carcinoma, suggesting that SCP-1 might be a new tumor target antigen of nasopharyngeal carcinoma.

关 键 词：鼻咽肿瘤 癌-睾丸抗原 SCP-1 聚合酶链反应 

分 类 号：R765.2[医药卫生—耳鼻咽喉科]