西罗莫司和FTY720对成人胰岛细胞毒性作用的体外研究  

Toxicities of sirolimus and FTY720 on the human pancreatic islets in vitro

作　　者：蔡锦全[1] 谭建明[1] 董维平[2] 王煜菲[2] 王鉴波[2] 

机构地区：[1]南京军区福州总医院泌尿外科全军器官移植中心,福州 350025 [2]上海市第一人民医院糖尿病研究室

出　　处：《中华器官移植杂志》2007年第4期226-228,共3页Chinese Journal of Organ Transplantation

基　　金：福建省重大科技项目（2002Y007）;全军重大课题（2004Z007）

摘　　要：目的 探讨西罗莫司（SRL）和FTY720在体外培养试验中对成人胰岛细胞的毒性作用。方法 取成人尸体胰腺，用Liberase胶原酶消化，采用Ficoll密度梯度法纯化胰岛细胞。再将其分别与不同浓度的FTY 720（3、6、15μg／L）和SRL（3、6、15μg／L）共同培养，并设不用任何药物的对照组。各组用低糖和高糖刺激胰岛素分泌，以酶联免疫（ELISA）法检测各培养液中胰岛素的含量，并进行比较分析。结果 各组在高糖刺激下的胰岛素分泌量均大于低糖刺激下的胰岛素分泌量（P〈0．05）。在低糖和高糖刺激时，FTY720和SRL之间及同一免疫抑制剂不同浓度之间的胰岛素分泌量相比较，差异均无统计学意义（P〉0．05）；各组与对照组比较，差异亦无统计学意义（P〉0．05）。结论 FTY720和SRL对成人胰岛细胞无明显的毒性作用。FTY720和SRL可作为临床胰岛细胞移植后的主要免疫抑制剂。Objective To investigate the toxicities of Sirolimus （SRL） and FTY720 on human pancreatic islets in vitro. Methods After procurement, cadaveric pancreases were digested by Liberase solution and pancreatic islets were purified by Ficoll＇s density gradient centrifugation. Islets were exposed to various concentrations of FTY720 （3, 6, 15μg/L） and Sirolimus （3, 6, 15 μg/L）, and compared with blank controls. Glucose-stimulated insulin secretion during subsequent static incubation was measured by the human insulin ELISA kit. Results High glucose-stimulated insulin secretion was significantly improved as compared with low glucose-stimulated one （P〈0.05）. No difference in insulin secretion was observed among FTY720, Sirolimus or blank control group （P〉0. 05）. Conclusion Without detectable toxicities on insulin secretion in human pancreatic islets, sirolimus and FTY720 could act as primary immunosuppressants for future clinical islet allotransplantation.

关 键 词：免疫抑制剂 胰岛移植 

分 类 号：R686[医药卫生—骨科学]