吗啡成瘾性大鼠脑内核团神经细胞[Ca^(2+)]i变化的研究  

The changes of neuroal intracellular free calcium in brain regions of morphine-addicted rats

作　　者：王林[1] 傅先明[1] 钱若兵[1] 牛朝诗[1] 汪业汉[1] 鲁明[1] 

机构地区：[1]安徽医科大学附属省立医院神经外科,合肥230001

出　　处：《立体定向和功能性神经外科杂志》2007年第2期84-87,共4页Chinese Journal of Stereotactic and Functional Neurosurgery

摘　　要：目的研究吗啡成瘾对大鼠脑内与成瘾相关核团神经细胞[Ca2+]i的影响。方法将50只SD大鼠随机分成吗啡成瘾组和对照组,观察成瘾后的戒断症状;应用对Ca2+敏感的探针Fluo-3与Ca2+络合后被激光激发发出荧光的特性,利用激光共聚焦显微镜观察伏核、海马、内侧额前皮质神经细胞[Ca2+]i变化,并用图像分析软件进行处理。结果吗啡成瘾大鼠脑内伏核、海马及内侧额前皮质神经细胞[Ca2+]i和对照组比较明显增高(P<0.01)。结论长期使用吗啡可明显增高伏核、海马和内侧额前皮质神经细胞[Ca2+]i。Objective To investigate the effects of morphine on the changes ot neuroal intracellular free calcium in brain regions of rat. Methods 50 male Spraqui-Dawlay rats were randomly assigned to experimental or control groups. Changes of [Ca^2＋]i in nucleus accumbens, hippocampus and medial prefrontal cortex were detected with laser scanning confocal microscope （LSCM） by using Ca^2＋ -sensitive dye fluo-3 as a calcium fluorescent probe. Results [Ca^2＋] i in the nucleus accumbens, hippocampus and medial prefrontal cortex were increased in morphine -addicted rats than that of control （P〈0.05）. Conclusion Long-term morphine exposure can lead to damage in different brain regions and increase of neuroal [Ca^2＋]i.

关 键 词：吗啡成瘾 钙离子 伏核 海马 激光共聚焦显微镜 

分 类 号：R996[医药卫生—毒理学]