RP-HPLC法测定补髓生血颗粒中淫羊藿苷(C_(33)H_(40)O_(15))的含量  

Determination of Lcariin(C_(33)H_(40)O_(15))in Busui Shengxue Granules by RP-HPLC

作　　者：田原[1] 樊辉[1] 关开[1] 杨明[1] 庞贺[1] 卢春玲[1] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《辽宁中医药大学学报》2007年第3期191-192,共2页Journal of Liaoning University of Traditional Chinese Medicine

摘　　要：目的:建立补髓生血颗粒的含量测定方法。方法:采用RP-HPLC法测定补髓生血颗粒中淫羊藿苷(C33H40O15)的含量。使用C18柱,乙腈-甲醇-0.2mol/L磷酸水溶液(24∶9∶67)为流动相,检测波长为270nm。结果:淫羊藿苷的平均回收率为98.04%,RSD为1.9%。结论:方法稳定性、重复性好,结果准确、可靠。Objective ： To establish method for determining the Content of Busui Shengxue Granules. Methods ： The content of Lcariin was determined by RP-HPLC. Lcariin was separated on spherisorb C18 column by acetonitrile-methol-0.2mol/L phosphoric acetic （ 24 ： 9 ： 67 ） as the mobile phase, the detection wavelength was 270nm. Results ： The average recovery was 98.04% and RSD was 1.9%.Conclusions ： The method was highly reproducible and accurate.

关 键 词：补髓生血颗粒 淫羊藿苷 RP—HPLC 含量测定 

分 类 号：R284.1[医药卫生—中药学]