淮山的组织培养与快速繁殖  

Tissue culture and plantlet regeneration in vitro of Dioscorea fordii Prain et Burkill

作　　者：丰锋[1] 叶春海[1] 王耀辉[1] 李发钦 

机构地区：[1]广东海洋大学农学院,广东湛江524088 [2]广东廉江市平坦农技站,广东廉江524432

出　　处：《仲恺农业技术学院学报》2007年第1期24-28,32,共6页Journal of Zhongkai Agrotechnical College

基　　金：广东省科技厅农业攻关项目(2006B202010022);广东省农业厅农业科技项目(B05083)资助

摘　　要：以淮山（Dioscorea fordii Prain et Burkill）茎段为材料，以MS为基本培养基，在（25±1）℃、光照强度24～30umol/m^2·s^-1、光照时间10h／d条件下对淮山的组织培养与快速繁殖技术进行了研究．结果表明，组合MS＋NAA0.03mg/L＋KT3．00mg／L＋Ad5.00mg/L＋PVP100mg/L对芽的诱导最好，平均芽数达3.5445个，出芽率为94.45％；组合MS＋NAA0.01mg/L＋KT3.00mg/L＋Ad7.50mg/L＋PVP300mg/L对多芽体的增殖最好，平均增重2.9686倍；组合MS＋IBA0.20mg/L＋PVP300mg/L对生根最好，平均根数达15．5452条．Using steins as materials, propagation in vitro of Dioscorea fordii Prain et Burkill was studied under the temperature of （25 ± 1 ） ℃, illumination intensity of 24 - 30umol · m^- 2· s^-1 and illumination time of 10 h/d, in the MS basic culture medium. Results showed that combination of MS ＋ NAA 0.03 mg/L ＋ KT 3.00 mg/L ＋ Ad 5.00 mg/L ＋ PVP 100 mg/L was the best for induction of the bud. The average bud amount was 3.544 5 and the budding rate was 94.45%. The combination of MS ＋ NAA 0.01 mg/L＋ KT 3.00 mg/L ＋ Ad 7.50 mg/L ＋ PVP 300 mg/L was the best for multiple-buds production, average produced multiple-buds was 2.968 6. Combination of the MS ＋ IBA 0.20 mg/L ＋ PVP 300 mg/L was the best for rooting, and the average mot number was 15.545 2.

关 键 词：淮山(Dioscorea fordii Prain ET Burkill.) 组织培养 多芽体 

分 类 号：Q943.1[生物学—植物学] S632.1[农业科学—蔬菜学]