咖啡酸锗抗肝癌作用及其机制的实验研究  

Empirical Study on the Effect and Mechanism of Caffeic Acid Germanium to Hepatocarcinoma

作　　者：黄越燕[1] 隋燕霞[2] 肖纯[3] 黄桂林[3] 刘春花[3] 周志愉[3] 杨波[1] 

机构地区：[1]浙江大学药学院,杭州310031 [2]西安交通大学医学院一附院,西安710061 [3]江西中医学院,南昌330006

出　　处：《江西中医学院学报》2007年第2期61-63,共3页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.30460148)

摘　　要：目的:探讨咖啡酸锗对H22肝癌小鼠的体内抗肿瘤作用及其诱导细胞凋亡的机制。方法:采用H22肝癌小鼠模型观察咖啡酸锗的体内抗肿瘤作用,透射电镜观察肿瘤细胞超微结构改变,免疫组化方法检测肿瘤组织中bax、bcl-2蛋白表达。结果:咖啡酸锗对小鼠H22移植瘤的生长具有明显抑制作用,高、中浓度组抑瘤率分别为57.0%、40.4%(P<0.01),电镜下可见典型的细胞凋亡形态特征。咖啡酸锗处理后肿瘤组织中bax蛋白表达明显增强,bcl-2表达降低(P<0.05)。结论:咖啡酸锗对H22肝癌小鼠具有明显的体内抗肿瘤作用,同时能够促进肿瘤组织中bax蛋白表达,抑制bcl-2蛋白表达,从而诱导肿瘤细胞凋亡。Objective： To approach the effect of caffeic acid germanium to H22 hepatoma mouse and the mechanism of inducing cell apoptosis. Methods： Observe the effect of caffeic acid germanium to H22 hepatoma mouse in vivo, observe the ultramicro- structural change of tumor cell with transmission electron microscope, detect to the expression of bax, bcl- 2 protein in tumor tissue with immunohistochemistry method. Results： There has the manifest depressant effect of caffeic acid germanium to the growth of mouse H22 hepatoma, the rate of restraining tumor of high and middle density group is 57.0 %, 40.4 % （P 〈 0.01 ）. It is thus evident that morphologic characteristics of typical apoptosis in electron microscope, The expression of bax protein is increased and the expression of bcl - 2 is decreased in the tumor tissue has been deal with caffeic acid germanium（P〈 0.05）. Conclusion： There is evident effect of caffeic acid germanium to H22 hepatoma mouse in vivo, at the same time, it can prompt the expression of bax protein in tumor tissue and restrain the expression of bcl - 2 protein, inducing the apoptosis of tumor cell.

关 键 词：咖啡酸锗 肝癌 H22荷瘤小鼠 细胞凋亡 抗肿瘤 

分 类 号：R965[医药卫生—药理学]