壳聚糖诱导黄瓜幼苗抗灰霉病  

Induced resistance of cucumber seedlings to Botrytis cinerea Pers.by chitosan treatment

作　　者：尚庆茂[1] 张志刚[1] 宋士清[2] 李晓芬[1] 董涛[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]南京农业大学园艺学院,南京210095

出　　处：《植物保护学报》2007年第2期136-140,共5页Journal of Plant Protection

基　　金：国家科研院所技术开发研究专项(2005-GKZX-316);国家科技攻关计划项目(2004BA521B01);农业部蔬菜遗传与生理重点开放实验室资助项目

摘　　要：为了明确壳聚糖(chitosan,CTS)对黄瓜幼苗抗灰霉病的诱导作用,采用根际注射结合叶面喷洒的方法,测定了灰霉病菌侵染下幼苗植株病情指数、防御酶活性和抗病相关物质含量等生理指标。结果表明:CTS降低了黄瓜幼苗的病情指数,最高幅度达39.4%,提高了苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)和多酚氧化酶(PPO)活性,增加了总酚、类黄酮和木质素含量。说明CTS能诱导黄瓜幼苗对灰霉病的抗性,且最佳处理浓度为200mg·L-1。Root injection and foliar spray were adopted to study induced resistance and physiological and biochemical mechanisms of ehitosan on Botrytis cinerea Pers. of cucumber, Cucumis sativus L. , and disease index, defense enzyme activities and disease resistance-related substance contents in cucumber seedlings were determined. The results showed that chitosan decreased disease index of cucumber seedlings by 39.4% , increased phenylalanine ammonia lyase （PAL） , peroxidase （POD） and polyphenol oxidase （PPO） activities, enhanced total polyphenol, flavonoid and lignin content. Chitosan played an important role in inducing disease resistance of cucumber seedlings with the optimum concentration at 200 mg·L^-1.

关 键 词：黄瓜 壳聚糖 灰霉病 诱导抗性 

分 类 号：S436.421[农业科学—农业昆虫与害虫防治]