茶毛虫核型多角体病毒Bt混剂的作用特性  

The effect specialty of EpNPV-Bt preparation

作　　者：冷杨[1] 肖强[1] 殷坤山[1] 

机构地区：[1]中国农业科学院茶叶研究所

出　　处：《植物保护学报》2007年第2期177-181,共5页Journal of Plant Protection

基　　金：国家"十五"科技攻关项目(2001BA5021302);浙江省科技计划(2003C32003)

摘　　要：用生物测定的方法,研究了茶毛虫核型多角体病毒(EpNPV)与苏云金杆菌(Bt)配制而成的茶毛虫核型多角体病毒Bt混剂(EpNPV-Bt)的增效作用、速效作用、拒食作用和兼治作用。结果表明,在16～28℃温度范围内,EpNPV-Bt对茶毛虫的作用在整个死亡周期表现为相加作用,协同毒力指数主要分布在-20%～20%之间,其中16、19、28℃的死亡前期表现为增效作用,协同毒力指数大于20%,22℃的死亡中期表现为拮抗作用,协同毒力指数小于-20%。EpNPV-Bt对茶毛虫LT30为5.6天,比EpNPV减少3.8天,幼虫的死亡速度提高了1.8倍。EpNPV-Bt对茶毛虫的拒食效果明显,取食量比EpNPV减少66.2%。EpNPV-Bt对茶刺蛾、用克尺蠖和茶尺蠖等茶树害虫具有兼治作用,其中对茶刺蛾的死亡率达85.8%。Bioassay were conducted to study the synergism, immediate functioning, anti-feeding effect and dual effect of EpNPV-Bt, the mixture of Euproctis pseucloconspersa Strand nuclear polyhedrosis virus （EpNPV） and Bacillus thuringiensis （Bt）. Under the temperature ranged from 16℃ to 28℃, the results showed that the EpNPV-Bt against the larvae of Euproctis pseudoconspersa Strand possessed joint action at the whole death period, the dynamic of synergic toxicity was mostly distributed between -20% to 20%. Hereinto at the death initial period of the 16℃ 19℃ and 28℃ treatments, the dynamic of synergic toxicity was more than 20% which showed synergism. At the death middle period of 22℃ treatment, the dynamic of synergic toxicity was less than -20%, which showed antagonism. Compared with EpNPV, the immediate functioning and anti-feeding effect of the EpNPV-Bt was evident. The LT30 （30% lethal time） of the EpNPV-Bt against the larvae of Euproctis pseudoconspersa Strand was lessened from 9.4 d to 5.6 d, and the food intake was decreased to 66.2%. It also showed a good dual effect of the EpNPV-Bt to control the larvae of Iragoides fasciata （Moore）, Jankowskia athleta Oberthur and Ectropis oblique Warren which are the main tea pests. The mortality of the EpNPV-Bt against the larvae of Iragoides fasciata （Moore） was up to 85.8%.

关 键 词：茶毛虫核型多角体病毒 苏云金杆菌 混剂 增效作用 

分 类 号：S435.711[农业科学—农业昆虫与害虫防治]