血红素氧化酶-1在急性镉中毒大鼠肾皮质中的表达  

Expression of Heme Oxygenase-1 in Renal Cortex of Rats Acutely Poisoned by Cadmium

作　　者：韦艳宏[1] 李龙[1] 曹玉广[1] 石年[1] 戴中华[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院卫生毒理学系和教育部环境与健康重点实验室,湖北武汉430030

出　　处：《癌变．畸变．突变》2007年第2期133-135,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：湖北省自然科学基金(No2002AB123)

摘　　要：背景与目的:观察急性镉中毒大鼠肾皮质中血红素氧化酶-1(heme oxygenase-1,HO-1)表达的变化。材料与方法:健康雄性SD成年大鼠24只,随机分为4组,3个染镉组连续5d分别腹腔注射0.4、0.8、1.6mg/kg氯化镉(CdCl2);对照组连续5d腹腔注射生理盐水。给药结束后5d取双肾皮质分别进行RT-PCR及免疫组织化学分析,观察HO-1mRNA及蛋白表达的变化。结果:对照组观察到HO-1的生理性表达。在mRNA水平,0.4mg/kg组未见表达增加,0.8及1.6mg/kg组较对照组明显升高(P<0.05)。mRNA与染镉剂量呈剂量-效应关系(r=0.97,P<0.05)。免疫组化结果显示,肾皮质HO-1蛋白表达的变化较mRNA敏感。在蛋白水平,3个试验组与对照组相比,差异均有统计学意义(P<0.05)。结论:镉的急性毒性作用可诱导肾脏表达HO-1增高,其表达可能具有保护作用。BACKGROUND ＆ AIM： To observe the changes of heme oxygenase-1 （HO-1） expressed in renal cortex of rats with acute cadmium exposure. MATERIALS AND METHODS： 24 male SD rats were divided randomly into 4 groups. There were a control group and the three experimental groups received daily intraperitoneal injections of CdCl2 （0.4, 0.8, 1.6 mg/kg） for five days. 0.9% NaCl substituted for CdCl2 in control. The specimens of kidney were prepared to measure the expression of HO-1 by RT-PCR and immunohistochemistry technique on the fifth day after injection. RESULTS： The physical expression of HO-1 was detected in control group. The levels of expressed mRNA in 0.8 and 1.6 mg/kg groups increased dramatically （P 〈 0.05） compared with the control while the mRNA concentration of 0.4 mg/kg group was equal to the control. A dose-effect relationship between the cadmium exposure and expression of HO-1 mRNA（r = 0.97, P 〈 0.05） was also found. Analysis of immunohistochemistry showed that changes of protein expression was more sensitive than mRNA in all three groups compared with the control（P〈0.05） . CONCLUSION： The acute exposure to cadmium could induce HO-1 expression in renal cortex of rats and this might provide potential protection.

关 键 词：镉 肾 HO-1 MRNA 免疫组织化学 

分 类 号：Q786[生物学—分子生物学]