神经生长因子与睫状神经营养因子促进大鼠坐骨神经再生的协同作用研究  

Research of synergistic effects of regenerative nerve fibers affected by nerve growth factor and ciliary neurotrophic factor in rats

作　　者：李强[1] 伍亚民[2] 申屠刚[1] 李民[1] 

机构地区：[1]解放军第98医院南京军区显微骨科中心,浙江湖洲313000 [2]第三军医大学创伤外科研究所

出　　处：《中国骨伤》2007年第4期220-223,共4页China Journal of Orthopaedics and Traumatology

基　　金：国家重大基础研究发展规划(973)项目(G1999054206)

摘　　要：目的:利用新型神经再生小室探讨神经生长因子(nerve growth factor,NGF)和睫状神经营养因子(ciliary neurotrophic factor,CNTF)促进周围神经再生的协同性作用。方法:36只大鼠随机分为A、B、C3组,每组12只,用自行研制的梭形双通道桥接管桥接坐骨神经10mm缺损。A组,2支管内均注入几丁糖凝胶(100μl);B组,一侧支管内注入几丁糖(100μl)+NGF(5μl)+生理盐水(5μl),另一侧支管内注入几丁糖(100μl)+NGF(5μl)+CNTF(5μl);C组,一侧支管内注入几丁糖(100μl)+CNTF(5μl)+生理盐水(5μl),另一侧支管内注入几丁糖(100μl)+NGF(5μl)+CNTF(5μl)。术后8、16周对再生神经的大体形态、常规病理及超微结构进行观察,并用核黄逆行示踪评估再生神经的轴浆运输功能。结果:NGF+CNTF侧再生神经呈淡黄色,质韧,弹性佳,优于对照侧,形态学观察见再生神经纤维数目多,粗细均匀,排列整齐,有髓纤维髓鞘厚,轴突直径大。其轴浆运输功能也明显优于对照侧。结论:NGF与CNTF对促进周围神经形态结构和功能的恢复均具有明显的协同作用。Objective： To discuss the synergistic effects on regenerative nerve fibers affected by nerve growth factor （NGF） and ciliary neurotrophic factor（CNTF） with the new cabin of peripheral nerve regeneration. Methods ： Thirty-six Sprague-Dawley rats with 10 mm gap of sciatic nerve were averaged divided randomly into three groups（ A, B, C）,which had been bridged with the new double channel nerve conduit of fusiform shape. In group A, 100μl chitin for medical use was injected into the two branches of the conduit ;in group B, beside injection chitin, 5 μl NGF and normal saline, 5 μl NGF and CNTF were respectively injected into the two branches; in group C, beside injection chitin,5 μl CNTF and normal saline, 5 μl NGF and CNTF were respectively injected into the two branches. At 8 and 16 weeks after operation, the morphology and ultrastructure of regenerative nerve were evaluated by HE staining and electron microscope. The function of axoplasmic transport was analyzed by nuclear yellow. Results：The regenerative nerve in the side where NGF and CNTF were injected were light yellow, and the resilient is also senior to the ones in the control side. The diameter of the regenerative nerve fibers in the synergistic side is coincident and line up in order under light microscope, the number of myelinated or unmyelinated fibers are more, the thickness of myelinated fibers is larger, the function of axoplasmic transport was also better than the nerve in thd control sides. Conclusion：There is significant synergistic effect of NGF and CNTF on promoting the regeneration of peripheral nerve.

关 键 词：神经生长因子 睫状神经营养因子 周围神经 神经再生 大鼠 

分 类 号：R651.3[医药卫生—外科学]