合浦珠母贝遗传连锁图谱的构建  

Construction of genetic linkage maps in pearl oyster Pinctada fucata using AFLP markers

作　　者：喻达辉[1] 王小玉[1] 黄桂菊[1] 郭奕惠[1] 龚世园[2] 王爱民[3] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]华中农业大学水产学院,湖北武汉430070 [3]海南大学海洋学院,海南海口570228

出　　处：《中国水产科学》2007年第3期361-368,共8页Journal of Fishery Sciences of China

基　　金：国家863基础研究计划(2002AA603022);广东省科技计划(2002B2150101);广东省自然科学基金(037148)

摘　　要：用AFLP标记构建了印度合浦珠母贝(Pinctada fucata)全同胞家系的遗传图谱。用经过筛选的36对引物组合对父母本和62个子代个体进行遗传分析,共得到1 547个标记,包括581个1∶1分离标记。母本分离标记294个,其中178个符合1∶1孟德尔分离规律;父本分离标记287个,其中182个符合1∶1孟德尔分离规律。雌性框架图包括33个遗传标记,分布在14个连锁群中,标记间平均间隔24.3 cM,有2个3联体,11个连锁对,图谱总长度为488.5 cM。雄性框架图包括53个遗传标记,分布在19个连锁群中,标记间平均间隔30.5 cM,有4个3联体,10个连锁对,图谱总长度为1 035.5 cM。雌雄两个框架图中AFLP标记的分布都较均匀。雌雄基因组估算长度分别为1 168.4 cM和2 037.1 cM,图谱覆盖率分别为41.8%和50.8%。本研究为进一步构建高密度遗传连锁图谱及QTL定位分析奠定了基础。Pearl oyster Pinctada fucata is the most important species cultured for pearl production in China. It is cultured mainly along the coasts of Guangdong, Guangxi and Hainan Provinces in south China. Yet great mortalities occurred frequently in recent years, possibly due to genetic degradation. Thereby, a selective breeding program has been initiated for genetic improvement of this animal, including family selection. In this study, male and female linkage maps were constructed preliminarily using AFLP markers based on an Indian family of Pinctada fucata. A total of 1 547 markers were generated in two parents and 62 progenies of the mapping family by using 36 screened AFLP primer combinations. Among the 1 547 markers, 581 were polynSorphic between the parents. Chi square analysis indicated that among the 294 female and the 287 male segregating markers 178 （60.5%） female and 182 （63.4%） male markers were in agreement with the Mendelian segregating ratio of 1 ： 1, based on which the female and male genetic linkage maps were constructed. The female framework map was composed of 33 segregating markers in 14 linkage groups （LGs） including 2 triplets and 11 doublets, covering a total of 488.5 cM with the average interval of 24.3 cM between markers. The male framework map consisted of 53 markers in 19 LGs including 4 triplets and 10 doublets, spanning 1 035.5 cM with the average interval of 30.5 cM. The estimated genome length of the pearl oyster was 1 168.4 cM for the females and 2 037.1 cM for the males,and the observed coverage was 41.8 % for the female map and 50.8 % for the male map. The genetic linkage maps demonstrated that the AFLP markers appeared to be evenly distributed within the linkage groups. These maps, though preliminary, provide a starting point for further construction of denser maps,as well as for the mapping of functional genes and quantitative trait loci（QTL） in P. fucata. [Journal of Fishery Sciences of China,2007,14（3） ： 361 -368]

关 键 词：合浦珠母贝 遗传连锁图谱 AFLP标记 

分 类 号：Q959.213[生物学—动物学]