抑制性消减杂交技术在养殖鱼类免疫基因克隆中的应用  

Suppression subtractive hybridization and its application in cloning immune-related genes from cultured fish

作　　者：王传娟[1] 张晓华[1] 贾爱荣[1] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《中国水产科学》2007年第3期504-512,共9页Journal of Fishery Sciences of China

基　　金：教育部新世纪优秀人才支持计划(NCET-04-0646);教育部留学回国人员科研启动基金(2005383)

摘　　要：抑制性消减杂交技术(SSH)是近年来兴起的一种分离、克隆差异基因的新技术,它结合了消减杂交和抑制PCR的优点,具有操作简便、特异性强、背景低及重复性好的优点。目前已经用SSH技术鉴定出了鱼的许多免疫相关基因,如白细胞介素、趋化因子、肿瘤坏死因子、溶菌酶、NKEF、补体、干扰素及急性期蛋白基因等,对它们的结构和功能进行了较为深入的研究。本文对SSH技术在养殖鱼类中克隆的免疫基因进行了归纳与总结,旨为全面了解鱼类的抗病免疫基因提供基础资料。Suppression subtractive hybridization （SSH） is one of the most powerful methods for isolating differentially expressed transcripts, and it has been employed in identification of immune genes of hosts in relation to infections. To date, researchers have applied SSH method to investigate many differential expressed genes in inflammatory response, including genes encoding interleukins （ILs）, chemokine, tumor necrosis factor （TNF）, lysozyme, natural killer cell enhancing factors （NKEF）, complement, interferon （IFN）, acute phase proteins （APPs）, etc, and the structure and function of the genes have been studied. The screening for immune-related functional genes in fish is very important for identifying the molecular mechanism for fish disease resistance. [Journal of Fishery Sciences of China, 2007,14 （3） ： 504 - 512 ]

关 键 词：抑制性消减杂交技术 SSH 水产养殖鱼类 基因克隆 免疫基因 

分 类 号：S91[农业科学—水产科学]