海洛因依赖期间大鼠胃窦5-羟色胺免疫反应细胞形态和功能变化  

Studies on Morphological and Functional Changes of Hydroxytryptamine Immunoreactive Cells in Gastric Antrum in Heroin Dependent Rats

作　　者：夏白娟[1] 梁文妹[1] 

机构地区：[1]贵阳医学院组织学与胚胎学教研室,贵州贵阳550004

出　　处：《贵阳医学院学报》2007年第2期131-134,共4页Journal of Guiyang Medical College

基　　金：贵州省优秀科技教育人才省长专项资助项目[黔科教办(2001-3)];贵州省教育厅科研基金资助项目[黔教科(1999)]

摘　　要：目的:探讨胃窦5-羟色胺(5-HT)免疫反应细胞在海洛因依赖期间的作用。方法:应用免疫组织化学SABC法及图像分析方法研究海洛因依赖期间大鼠胃窦5-HT-IR细胞平均灰度值和细胞计数的变化。结果:与正常组及盐水组比较,海洛因依赖组大鼠胃窦5-HT-IR细胞数量显著增加(P<0.01),细胞的平均灰度值较正常和盐水对照组显著降低,在第24天时变化最为明显(P<0.01)。结论:大鼠胃窦5-HT-IR细胞通过增加细胞数和增强5-HT的分泌,参与了机体在海洛因依赖期的调节过程。Objective： To explore the possible function of hydroxytryptamine immunoreactive （5-HT- IR） cells in gastric antrum of rats during heroin dependence. Methods： Immunohistochemical SABC method and imagine analysis were used to observe the changes of the mean gray degree and number of 5-HT IR cells in gastric antrum of rats during heroin dependence. Results： As compared with rats in normal control group and saline control group, the number of 5-HT-IR cells increased （ P 〈 0.01 ）, and the mean gray degree of 5-HT-IR cells decreased in rats of heroin dependent group. The peak of these changes occurred on the 24th day. Conclusions： 5-HT-IR cells might involve in the body physiological regulation during heroin dependence by increasing the cell numbers and enhancing the synthesis of hydroxytryptamine.

关 键 词：海洛因依赖 胃窦 5-羟色胺 免疫组织化学 大鼠 

分 类 号：R329.44[医药卫生—人体解剖和组织胚胎学] R996[医药卫生—基础医学]