心脏复苏后犬血清炎症指标的变化  

Alteration of serum Inflammatory Markers in Resuscitated Dog after Cardiac Arrest

作　　者：李燕屏[1] 江慧琳[1] 陈晓辉[1] 许松青[1] 张弋[1] 

机构地区：[1]广州医学院第二附属医院急诊科,广东广州510260

出　　处：《中华高血压杂志》2007年第4期315-318,共4页Chinese Journal of Hypertension

基　　金：广东省卫生厅资助项目(A2003315)

摘　　要：目的观察心脏复苏后犬血清及心肌肿瘤坏死因子α(TNF-α)、白介素6(IL-6)、白介素10(IL-10)的变化及临床意义。方法随机对致室颤犬行心肺复苏,6只恢复心跳入复苏组,6只不致颤为对照组。测室颤前、心脏复跳0、1、2、4、6h血清TNF-α、IL-6、IL-10浓度及其6h在心肌的表达,同时监测心排量等。结果血清TNF-α从0h[(5.5±1.3)pg/mL]、IL-6从2h[(12.4±7.7)pg/mL]开始显著升高(P<0.05),至6h均达到最高[TNF-α(30.3±14.4)pg/mL;IL-6(26.8±6.5)pg/mL,P均<0.01],IL-10测不到。6h心肌TNF-α、IL-6、IL-10 mRNA表达增加显著(P<0.01)。6h心排量最低。结论恢复自主循环2h内血清TNF-α、IL-6浓度先后升高,IL-10滞后,提示机体启动了全身炎症反应。其中心肌炎症反应是复苏后心功能不全原因之一,血清TNF-α、IL-6浓度越高心功能越差。Objective To investigate the change of the serum inflammatory markers such as tumor necrosis factor-α（TNF-α）, interleukin-6 （IL-6） and interleukin-10 （IL-10） and the expression of these inflammatory markers mRNA in myocardium in postresuscitation dog. Methods Twelve dogs were randomly divided into the control groups （n=6） and cardiopulmonary resuscitation（CPR） group（n=6）. TNF-α, IL-6 and IL-10 were determined before and after successful resuscitation. Results The serum level of TNF-α, IL-6 in CPR dogs increased gradually and higher than those in the control dogs （TNF-α, 0 h： 5.5±1.3 vs 6 h： 30. 3±14.4 pg/mL; IL-6, 2 h： 12.4±7.7 vs 6 h 26.8±6.5 pg/mL, P〈0.01）. The mRNA expression of myocardial TNF-α, IL-6 and IL- 10 in CPR dogs was significantly higher than that in control group. Conclusion There are systemic inflammatory response syndrome（SIRS） in post-resuscitation dogs after cardiac arrest which was associated with increases in expression of cytokines mRNA in myocardium.

关 键 词：心肺复苏 肿瘤坏死因子Α 白介素6 白介素10 

分 类 号：R541.7[医药卫生—心血管疾病]