乳大鼠心室肌培养细胞代替急性酶分离细胞用于起搏电流电生理研究的可行性探索  

The Feasibility Exploration of Cultured Newborn Rat Ventricular Myocyte Taking the Place of Acutely Enzymatic Isolated Myocardium for Study in Electrophysiology of Pacemaker Current

作　　者：贾卫国[1] 邓珏林[1] 廖大清[2] 李妙龄[3] 曾晓荣[3] 黄德嘉[4] 

机构地区：[1]四川大学华西医院老年科,成都610041 [2]四川大学华西医院麻醉与危重医学实验室 [3]四川泸州医学院心肌电生理实验室 [4]四川大学华西医院心内科

出　　处：《四川大学学报（医学版）》2007年第3期447-450,512,共5页Journal of Sichuan University(Medical Sciences)

摘　　要：目的探讨体外培养细胞代替急性酶分离细胞用于心室肌电生理研究的可行性。方法采用膜片钳全细胞记录技术,对比分析心室肌细胞培养的不同时间与急性酶分离的心室肌细胞起搏电流间的电生理学差异。结果短期的培养就会使细胞膜电容下降,各期培养细胞的膜电容小于急性酶分离细胞的膜电容〔(56±7)pF,P<0.05〕。4d后膜电容随着培养时间的延长有逐渐增大的趋势,但各期之间的膜电容差异无统计学意义(P>0.05)。各个培养时间段细胞的反转电位、电流密度、活化阈值、半最大激活膜电压(V0.5)等主要电生理指标之间差异无统计学意义(P>0.05)。结论至少短期培养的乳大鼠心室肌细胞的起搏电流与急性酶分离细胞的起搏电流的主要电生理指标之间无明显差异,可以代替急性分离细胞应用于起搏电流的电生理研究。Objective To investigate the feasibility of cultured rat ventricular myoeytes taking the place of acutely enzymatic isolated myocardia for study in the pacemaker current electrophysiology. Methods By using patch-clamp technique, the whole-cell pacemaker currents were recorded to study the difference of pacemaker current electrophysiology between neonatal rat ventricular myocytes ;.solated acutely and maintained in cell culture. Results The cell membrane capacitances （Cm） were significantly decreased in myocardia even upon short term of cell culture. The Cm of myocardia cultured 1-3 day were （43± 3） pF,with decreased 23% compared to acute enzymatic isolated cells [（56±7） pF, P〈0. 013. It seemed like the Cm increased slightly upon cell cultured time, but no significant difference appearing among various culture stages （P〉 0. 05） . No significant difference of pacemaker current electrophysiological character, such as reverse potential, current density, active threshold and V0.5, was detected from different cell group （P〉0. 05）. Conclusion The cultured myocardium can take place of acute enzymatic isolated myocardium used to study in pacemaker current. The cells cultured no more than one week may be more suitable to this kind of electrophysiological experiment.

关 键 词：哇巴因 起搏电流 膜片钳全细胞记录技术 心室肌细胞 

分 类 号：R331.38[医药卫生—人体生理学]