苦参碱对大鼠慢性环孢素肾毒性的保护作用的实验研究  

Experiment Research on Preventive Effect of Matrine on Rat Chronic Nephrotoxicity Induced by Cyclosporin A

作　　者：景宇[1] 白亚君[1] 陶冶[1] 付平[1] 吴欣[1] 李聃丹[1] 刘其峰[1] 

机构地区：[1]四川大学华西医院肾脏内科,成都610041

出　　处：《四川大学学报（医学版）》2007年第3期451-455,共5页Journal of Sichuan University(Medical Sciences)

摘　　要：目的探讨苦参碱对大鼠慢性环孢素A(CsA)肾毒性是否具有保护作用及其可能机制。方法采用大鼠慢性CsA肾毒性模型,将56只SD大鼠随机分为对照组、CsA模型组(CsA20mg/kg)、安博维组(安搏维10mg/kg+CsA20mg/kg)和苦参碱组(Matrine100mg/kg+CsA20mg/kg),经胃管灌胃,每天1次,实验共4周。于第2周和第4周末两次采集血尿标本进行生化检测,肾组织行常规病理检查和免疫组化检测骨桥蛋白(OPN)、Ectodermal Dysplasia1(ED-1)表达。结果与对照组比较,CsA组24h尿量、小管间质损伤评分、OPN表达和ED-1阳性细胞计数均增加,内生肌酐清除率(Ccr)下降(P均<0.05)。与CsA组相比,苦参碱能下调OPN表达,减少ED-1阳性细胞在肾组织的浸润,减轻肾脏病理改变(P<0.05)。结论苦参碱对实验性大鼠慢性CsA肾毒性具有一定的保护作用。Objective To investigate the renal protective effect and possible mechanism of matrine on experimental cyclosporine A （CsA） induced chronic nephrotoxicity. Methods 56 male Sprague-Dawley rats were randomly divided into four groups： control group （olive oil 0. 2 mL/d）, CsA group [CsA 20 mg/（kg · d）], Irbesartan group [CsA 20 mg/（kg · d） plus Irbesartan 10 mg/（kg · d）], matrine group [CsA 20 mg/（kg · d） plus matrine 100 mg/（kg · d）], and each group comprised fourteen rats. After treated with drugs, rats were sacrificed at the end of week 2 and 4. The body weight, 24-hours urine and blood sample were collected before rats sacrificed. The rat kidneys were taken and fixed in 10% neutral formaldehyde for HE staining. The osteopontin （OPN） and ectodermal dysplasia 1 （ED-1） were determined by immunohistochemical method. Results Compared to control group, the 24-hour urine of rats, histological scores, the expression of OPN and the ED-1 positive cells number of CsA group were significantly increased, but Ccr was significantly lower （P〈0. 05）. Matrine could down-regulate OPN expression and decrease ED-1 positive cell infiltration in kidney, compared with CsA group （P 〈0. 05）. Conclusion Matrine has a renal protective effect on chronic cyclosporine nephrotoxicity of rat model.

关 键 词：苦参碱 环孢素A 慢性肾毒性 骨桥蛋白 ED-1 

分 类 号：R285.5[医药卫生—中药学]