人血清白蛋白与锌离子缔合效应的中子小角散射研究  

THE ASSOCIATION EFFECT OF HUMAN SERUM ALBUMIN UNDER DIFFERENT ZINC ION CONCENTRATIONS STUDIED BY SMALL ANGIE OF NEUTRON SCATTERING

作　　者：贺健[1] 徐延平 杨同华[1] 贺进田[2] 李连之[2] 周永洽[2] 

机构地区：[1]中国原子能科学研究院核物理研究所 [2]南开大学化学系

出　　处：《原子能科学技术》1997年第2期168-172,共5页Atomic Energy Science and Technology

基　　金：核工业科学基金;国家自然科学基金(NSFC)资助

摘　　要：采用中子小角散射谱仪对系列溶液样品进行测量，直接获得锌离子－人血清白蛋白分子配位体的亚微观结构参数。结合平衡透析法实验结果和其它已知条件，定量地描述了锌离子与人血清白蛋白在不同条件下相互作用的微观过程，其结果与传统化学和生化方法所得结果相吻合。The microscopic parameters of the HSA Zn 2+ , R g and I (0),are obtained directedly by small angle of neutron scattering (SANS) under different concentrations of zinc ion.Comparing with other relevant experimental results,e.g.equilibrium dialysis,we can determine and distinguish the apparent interactive subsquences into more detailed sub actions, such as self association, inter assosciation,deintegration and deconformation et al.,or even certain combination of them,other than some indirect and microscopic results.Results obtained by SANS can qualitativewly and readily interpret the characteristics of from the viewpoint of microscopic process.

关 键 词：白蛋白 人血清白蛋白 锌离子 中子小角散射 

分 类 号：Q512.1[生物学—生物化学]