AAT蛋白在人精子中的鉴定和定位  

Identification and localization of AAT in human spermatozoa

作　　者：瞿飞[1] 郭崴[1] 郭强苏[1] 应晓倩[1] 丁之德[1] 

机构地区：[1]上海交通大学医学院组织胚胎学教研室上海市生殖重点实验室,上海200025

出　　处：《中国男科学杂志》2007年第4期36-38,共3页Chinese Journal of Andrology

摘　　要：目的对α1-抗胰蛋白酶(AAT)在人精液和精子中进行鉴定以及定位。方法应用免疫印迹方法鉴定AAT蛋白,利用激光共聚焦显微镜和间接免疫荧光定位的方法研究AAT在精子上的定位。结果证实在人精浆和精子里都存在AAT蛋白,且其主要定位于精子的头部,尤其在赤道板处有强荧光。结论通过对AAT在人精子上的鉴定和定位,可以帮助进一步研究该蛋白对精子的作用。Objective To identify and localize AAT in human semen, especially in spermatozoa. Methods AAT was identified by Western Blotting, and localized by indirect immunofluorescence studies using laser scanning confocal microscope. Results AAT was present in human semen and spermatozoa, which was mostly localized on the head region of spermatozoa, in particular on the equatorial plate. Conclusion This research will help further investigation of the possible relationship between AAT and human sperm.

关 键 词：AAT 精液 精子 

分 类 号：R446.144[医药卫生—诊断学]