Breeding for Male-Sterility Line with G. barbadense Nuclear Background and Cytological Observation of Its Microsporogenesis  

作　　者：ZHANG Xiao-quan WANG Xue-de ZHU Yun-guo ZHU Wei JIANG Pei-dong 

机构地区：[1]Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029 P.R.China

出　　处：《Agricultural Sciences in China》2007年第5期529-535,共7页中国农业科学（英文版）

摘　　要：To study the feasibility of utilizing the heterosis of interspecific （Gossypium barbadense x G. hirsutum） hybrid cotton based on cytoplasmic male-sterility system, a G. barbadense male-sterility line HaiA with G. harknessii cytoplasm and its maintainer HaiB were developed by replacing nuclear genome from G. hirsuturn to G. barbadense in a backcross breeding program. Its male cell abortion was observed using a light microscope through a paraffin slice for H.E staining technique. The results showed that the abortion characteristics in HaiA were not significantly changed at the cytological level, although G. hirsuturn nuclear genome of sterile line was replaced by G. barbadense nuclear genome, and its abortion mainly took place at the stage of the microspore mother cell meiosis. HaiA sterility was very stable because of its early abortion and no-pollens in anthers. Hybrids between HaiA and G. hirsuturn restorers were high in pollen viability, boll setting rate, boll weight and lint percentage which contributed to high yield heterosis. It is suggested that HaiA is of great value in the application of cotton interspecific hybrid seed production.To study the feasibility of utilizing the heterosis of interspecific （Gossypium barbadense x G. hirsutum） hybrid cotton based on cytoplasmic male-sterility system, a G. barbadense male-sterility line HaiA with G. harknessii cytoplasm and its maintainer HaiB were developed by replacing nuclear genome from G. hirsuturn to G. barbadense in a backcross breeding program. Its male cell abortion was observed using a light microscope through a paraffin slice for H.E staining technique. The results showed that the abortion characteristics in HaiA were not significantly changed at the cytological level, although G. hirsuturn nuclear genome of sterile line was replaced by G. barbadense nuclear genome, and its abortion mainly took place at the stage of the microspore mother cell meiosis. HaiA sterility was very stable because of its early abortion and no-pollens in anthers. Hybrids between HaiA and G. hirsuturn restorers were high in pollen viability, boll setting rate, boll weight and lint percentage which contributed to high yield heterosis. It is suggested that HaiA is of great value in the application of cotton interspecific hybrid seed production.

关 键 词：Gossypium barbadense cytoplasmic male sterility interspecific heterosis 

分 类 号：S562[农业科学—作物学]