Genetic Variations Among Liriomyza sativae Blanchard Populations Indicated by β-tubulin Gene Sequences  

Genetic Variations Among Liriomyza sativae Blanchard Populations Indicated by ?β-tubulin Gene Sequences

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作  者:ZHENG Fu-shan DU Yu-zhou WANG Li-ping LU Ya-juan LU Zi-qiang 

机构地区:[1]Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, P.R.China [2]Yangzhou Entry-Exit Inspection and Quarantine Bureau, Yangzhou 225009, P.R.China

出  处:《Agricultural Sciences in China》2007年第5期580-585,共6页中国农业科学(英文版)

基  金:funded by National Natural Science Foundation of China(30370932);973 Project of China(2006CB102002).

摘  要:To analyze the genetic differentiation of the host- and geo-populations of Liriomyza sativae Blanchard, the β-tubulin gene of 5 host-populations and 6 geo-populations of L. sativae was sequenced. Subsequently, the sequences were analyzed by biosoftware DNAStar and MEGA, and the phylogenetic trees constructed. The results obtained by the two softwares were similar, that is, the sequences of β-tubulin gene were more than 98% homologous, among the host- and geo-populations of L. sativae, with only 8 variable sites, but no insertions and deletions were detected. It seems that differentiations in β-tubulin gene among the host- and geo-populations of L. sativae are related to the hobby to hosts and the geographical distributions, respectively.To analyze the genetic differentiation of the host- and geo-populations of Liriomyza sativae Blanchard, the β-tubulin gene of 5 host-populations and 6 geo-populations of L. sativae was sequenced. Subsequently, the sequences were analyzed by biosoftware DNAStar and MEGA, and the phylogenetic trees constructed. The results obtained by the two softwares were similar, that is, the sequences of β-tubulin gene were more than 98% homologous, among the host- and geo-populations of L. sativae, with only 8 variable sites, but no insertions and deletions were detected. It seems that differentiations in β-tubulin gene among the host- and geo-populations of L. sativae are related to the hobby to hosts and the geographical distributions, respectively.

关 键 词:Liriomyza sativae Blanchard host-populations geo-populations β-tubulin gene genetic differentiation 

分 类 号:S433[农业科学—农业昆虫与害虫防治]

 

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