甲醛对植入后小鼠胚胎发育毒性的时间-效应关系  

Time-effect relationship of mice embryonic toxicity and teratogenicity induced by HCHO in vitro

作　　者：曹智丽[1] 李友[1] 贺彬琪[1] 李瑛[1] 王穆[1] 吴成秋[1] 陈锋[1] 让蔚清[1] 

机构地区：[1]南华大学公共卫生学院,衡阳421001

出　　处：《中国新药杂志》2007年第8期607-609,共3页Chinese Journal of New Drugs

基　　金：国家自然科学基金(30572191);湖南省卫生厅科研课题资助(B200508)

摘　　要：目的:探讨甲醛对植入后小鼠胚胎发育毒性及时间-效应关系。方法:应用体外全胚胎培养模型,观察终浓度为81μmol.L-1的甲醛与昆明种小鼠全胚胎共培养不同时间(分别为0,16,26,48 h)对胚胎生长发育的影响。结果:甲醛对小鼠胚胎的器官形成早期具有明显的影响,呈现明显的时间-效应关系。甲醛暴露16 h则能引起胚胎发育迟缓。当甲醛暴露时间为26和48 h时,胚胎生长发育和形态分化等各项指标与对照组(0 h)比较均呈现出显著差异(P<0.05,P<0.01);胚胎畸形发生率也显著升高,主要表现为神经管闭合不全、体位翻转不全、脑、心脏、腮弓发育异常及视觉系统、前肢芽、后肢芽、体节等畸形。结论:甲醛对小鼠胚胎生长发育和器官形态分化均有明显的抑制作用,表现出明显的时间-效应关系。Objective： To study the time-effect relationship of mice embryonic toxicity and terato- genicity induced by HCHO in vitro, nethods：Kunming mice embryos （8.5 days old） were incubated to- gether with formaldehyde （81μmol.L^-1）for 0, 16, 26 or 48 hours, respectively. The development of mice embryos was then assessed. Results：Formaldehyde degenerated early embryonic growth in a time- dependent manner. The embryonic development was significantly retarded post a 16-hours exposure of formaldehyde. The embryonic development and histomorphological differentiation were significantly sup- pressed post 26-and 48-hours exposure of formaldehyde, manifesting higher incidence rate of teratogenici- ty and embryonic dysmorphogenesis including neural tube defects, brain defects, abnormal heart, abnor- mal arch and small limb bud. Conclusion： Formaldehyde had a significantly conspicuous inhibitory ac- tion of embryonic development and morphological differentiation in a time-dependent manner.

关 键 词：甲醛 体外全胚胎培养 发育毒性 时间一效应关系 

分 类 号：R994.2[医药卫生—毒理学] R992[医药卫生—药学]