RP-HPLC法测定羧胺三唑含量及有关物质  

RP -HPLC determination of carboxyamidotriazole and related substances

作　　者：张俊松[1] 李翼[2] 戚燕[2] 王晓利[1] 吴松[2] 

机构地区：[1]深圳职业技术学院,深圳518055 [2]中国医学科学院中国协和医科大学药物研究所,北京100050

出　　处：《药物分析杂志》2007年第4期582-584,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用反相高效液相色谱法测定羧胺三唑的含量及其有关物质。方法:采用Kromasil C_(18)色谱柱(4.6mm×250mm,5μm);以乙腈-水相(45∶55)为流动相;流速2.0mL·min^(-1);检测波长:262nm;柱温:室温;进样量:20μL。结果:反相高效液相色谱法测定的线性范围为12.5～200μg·mL^(-1);相关系数r=0.9999;日内精密度:RSD为0.38%(n=5);日间精密度:RSD为0.39%(n=5)。结论:本法简便快速、准确、专属性好。Objective：A RP -HPLC method was established for the determination of Carboxyamidotriazole and its related substances. Method：The separation was performed on a Kromasil C18 column（4. 6 mm×250mm,5μm）. The mobile phase was composed of acetonitrile- water（45：55 ）. The flow rate was 2. 0 mL · min^-1. The detection was carried out with a UV detector at 262 nm, The chromatography was conducted at room temperature. The injection was 20 μL. Results：The calibration curves were linear between 12. 5 -200 g ·mL^-1（ r = 0. 9999 ）. Intra - day and inter - day RSD were 0. 38% （ n = 5 ） and 0.39% （ n = 5 ）. Conclusion： The method is simple, rapid, accurate and is suitable for the determination of Carboxyamidotriazole and its related substances.

关 键 词：羧胺三唑 反相高效液相色谱法 含量测定 有关物质 

分 类 号：R917[医药卫生—药物分析学]