反相高效液相色谱法测定8-甲氧补骨脂素乳膏的含量  

RP-HPLC determination of 8-methoxypsoralen cream

作　　者：颜宏生[1] 石善海[1] 田夫军[1] 马世尧[1] 

机构地区：[1]山东省皮肤病医院,济南250022

出　　处：《药物分析杂志》2007年第4期593-594,共2页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立一种反相高效液相色谱法测定8-甲氧补骨脂素乳膏中主药的含量。方法:采用以碳十八烷基键合硅胶柱为色谱柱,甲醇-水(70∶30)为流动相,检测波长为249nm的外标法测定含量。结果:8-甲氧补骨脂素在8～80μg·mL^(-1)(r=0.9999,n=5)浓度范围内呈良好线性关系,其平均回收率为98.26%～99.46%;RSD为0.50%～1.58%。结论:该方法简便、灵敏、快速、准确。Objective：To establish an RP - HPLC method for determination of 8 - methoxypsoralen cream. Methods：General C18 scolumn was adopted as stationary phase, and solvent mixture composed of methanol -water （70： 30） was used as mobile phase, the detection wavelength was 249 nm. Result：The method was fine linear over the range of 8 - 80 μg · mL^-1 （ r = 0. 9999, n = 5 ）, the average recovery and RSD respectively was 98.26% - 99.46% ,0.50% - 1.58%. Conclusion： It＇s a simple, sensitive, rapid, and reliable quantitative analysis method to 8 - methoxypsoralen cream.

关 键 词：反相高效液相色谱法 8-甲氧补骨脂素乳膏 

分 类 号：R917[医药卫生—药物分析学]