微生物发酵法降解山黧豆毒素的研究  

Studies on degrading neurotoxin ODAP in Lathyrus sativus by microbial fementation

作　　者：姚明明[1] 李志孝[2] 黄凯[1] 赵兴秀[1] 孟尧[1] 孟延发[1] 

机构地区：[1]四川大学生命科学学院,成都610061 [2]兰州大学化学化工学院,兰州730000

出　　处：《四川大学学报（自然科学版）》2007年第2期439-442,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(30270965)

摘　　要：为了寻找具有降解山黧豆神经毒素(ODAP)的菌株,本实验选择30种菌株,以含有已知量的ODAP的山黧豆粉提取液为培养基,采用邻苯二甲醛法检测培养前后培养基中ODAP的消耗量,筛选具有能够明显利用和消耗ODAP的菌株.实验结果表明,有2种菌株对ODAP有明显的降解和消耗作用.还探讨了这两种菌株在山黧豆培养基中的生长情况与ODAP降解情况的相关性以及酶促反应的基本动力学,从所获得的试验结果可断定被筛选出的2株菌种经发酵可产生ODAP降解酶.To screen the bacteria of degrading the neurotoxin ODAP from 30 kinds of bacteria by microbial fermentation,the consumed amounts of ODAP from Lathyrus sativus extraction as culture medium which contained fixed quantify of ODAP was determined phthaladehyde colorimetric method. The results showed that there were two kinds of bacteria which had the property of consuming obviously ODAP. In addition, the research of this paper included the relativity of the growth and the degradation of ODAP, and the enzyme kinetics of reaction velocity and time. Then it was concluded that it was capable of producing ODAP degrading enzyme by microbial fermentation in culture medium of ODAP.

关 键 词：山黧豆 β-N-草酰-L-α β-二氨基丙酸(ODAP) 微生物发酵法 ODAP降解酶 

分 类 号：Q93[生物学—微生物学]