湖南稻瘟病菌群体遗传多样性与病菌致病型的关系  

Genetic Diversity among Populations as Related to Pathotypes for Magnaporthe grisea in Hunan Province

作　　者：李亚[1] 刘二明[1] 戴良英[1] 李成云[2] 刘林[2] 

机构地区：[1]湖南农业大学生物安全科技学院,湖南长沙410128 [2]云南农业大学植物病理重点实验室,云南昆明650201

出　　处：《中国水稻科学》2007年第3期304-308,共5页Chinese Journal of Rice Science

基　　金：湖南省自然科学基金资助项目(13K0453);湖南省教育厅重点资助项目(06A029);湖南农业大学人才引进基金资助项目(60166:660410)

摘　　要：用8对SSR引物对230个稻瘟病菌菌株进行遗传多样性分析。经UPGMA聚类分析,在相异系数为0.23水平上,供试菌株划分为7个遗传宗谱,其中Ⅰ、Ⅴ、Ⅵ宗谱为优势宗谱,所占比例分别为23.9%、23.5%和23.0%,Ⅳ和Ⅶ宗谱中分别只有1个和5个菌株,其所占比例仅为0.43%和2.17%。用7个全国统一鉴别品种对从230个菌株中选出的77个菌株进行致病型鉴定,结果表明湖南稻瘟病菌分属ZA、ZB、ZC、ZD、ZE、ZF、ZG等共7群30个致病型。其中ZA、ZB、ZC 3个种群的分化较强,分别含6、10、7个致病型,其中ZG1为优势致病型,出现频率为26.5%。SSR标记的遗传多样性分析结果以及以不同鉴别品种表型来划分的致病型结果表明,菌株遗传宗谱与致病型不存在一一对应关系。The genetic diversity of 230 monoconidial isolates of Magnaporthe grisea was analyzed using eight pairs of SSR markers. All isolates tested were classified into seven genetic lineages at 0.23 level of the genetic dissimilarity through the clustering analysis of UPGMA. The lineages Ⅰ , Ⅴ , Ⅵ were the predominant ones with the rates of 23.9%, 23.5%, 23.0% respectively. Only one isolate fell into the lineage Ⅳ and five isolates belonged to the lineage Ⅶ. As 77 isolates selected from above monoconldial isolates were characterized with seven Chinese differential varieties, they were distributed to the 7 groups （ZA, ZB, ZC, ZD, ZE, ZF, ZG） and 30 pathotypes. There was great differentiation among ZA, ZB and ZC groups. ZG1 was the predominant race, and its frequency in the total blast fungus population was as high as 26.5 %. Compared with the results of SSR analysis and pathotypes, there wasn＇t obviously corresponding relationship between genetic lineages and pathotypes.

关 键 词：水稻 稻瘟病菌 微卫星标记 遗传多样性 致病型 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]