斑点金免疫渗滤法在布鲁氏菌病监测地区的现场应用  

Field application of dot immunogold filtration assay in brucellosis surveillance area

作　　者：朱明东[1] 徐卫民 洪林娣[1] 王衡 

机构地区：[1]浙江省医学科学院寄生虫病研究所流行病室,浙江杭州310013 [2]浙江省布病防治中心

出　　处：《疾病监测》2007年第4期220-221,共2页Disease Surveillance

基　　金：浙江省医药卫生科学研究基金资助(2004A004)

摘　　要：目的验证斑点金免疫渗滤法(DIGFA)在布鲁氏菌病监测地区的现场应用效果。方法选择布鲁氏菌病监测地区重点人群和不同职业人群,采用DIGFA和试管凝集试验(SAT)检测布鲁氏菌病抗体。结果重点人群两种方法检测抗体阳性符合率为96.66%,阴性符合率为99.92%;不同职业人群两种方法检测阳性符合率为96.36%,阴性符合率为99.92%,两者差异无统计学意义(P>0.05)。结论DIGFA与SAT同样具有敏感性强,特异性高等优点;此外,DIGFA还具有操作简便,快速,微量等优点,适合于布鲁氏菌病监测地区的现场应用。Objective This study was disigned to evaluate the field application effect of dot immunogold filtration assay （DIGFA） in brucellosis surveillance area. Methods The brucellosis antibody was detected by both DIGFA and serum agglutination test （SAT） among specific crowds and those of different occupation in the surveillance area. Results The positive antibody coincidence was 96.66%, negative coincidence 99.92% on detecting sera of specific persons by both the two methods, while the positive antibody coincidence was 96.36% and negative coincidence 99.92% among crowds of different occupation by both methods. There is no significant between DIGFA and SAT（P〉0.05）on detecting sera of specific crowds and those of different occupation. Condusion DIGFA and SAT are both sensitive and specific. Additionally, DIGFA is rapid, simple to operate, and capable of trace detection, and thus the proper approach to be applied in brucellosis surveillance area.

关 键 词：斑点余免疫渗滤法 布鲁氏菌病 监测地区 现场应用 

分 类 号：R516.7[医药卫生—内科学]