两步法PCR-CTPP技术在单核苷酸多态性研究中的应用  

The two-step polymerase chain reaction with confronting two-pair primers for genotyping in research of single nucleotide polymorphism

作　　者：刘勇[1] 王珍[1] 朱红[1] 陈江[1] 何春涤[1] 陈洪铎[1] 

机构地区：[1]中国医科大学附属第一医院,110001

出　　处：《山西医药杂志》2007年第5期391-393,共3页Shanxi Medical Journal

基　　金：国家十五重大科技专项:人类重大疾病相关基因的研究--重大疾病遗传资源的收集;保存与利用(2002BA711A08-28)基金资助

摘　　要：目的建立两步法聚合酶链反应(PCR)-CTPP技术体系,研究在单核苷酸多态性(SNP)等位基因分型中的应用价值。方法采用两步法PCR-CTPP技术检测180名正常个体p73基因第二外显子G4C14-A4T14多态性,并以PCR-RFLP和DNA测序验证基因分型结果。结果通过两步法PCR-CTPP得到的基因分型结果与PCR-RFLP和DNA测序得到的基因分型结果完全一致。结论两步法PCR-CTPP技术省时,结果稳定可靠,对于SNP等位基因分型具有较高的应用价值。Objective To evaluate the value of the two-step polymerase chain reaction with confronting two-pair primers （PCR-CTPP） for genotyping. Methods Two-step PCR-CTPP was utilized for detecting polymorphism of p73 exon 2 G4C14-A4T14 of 180 healthy controls and the same polymorphism was genotyped by PCR-restriction fragment length polymorphism （RFLP） and DNA sequence analysis. Results The results of genotyping in two-step PCR-CTPP were consistent with those conducted by PCR-RFLP and DNA sequence analysis. Conclusion The two-step PCR-CTPP is a fast and reliable method for genotyping.

关 键 词：聚合酶链反应 多态性 单核苷酸 P73基因 

分 类 号：R450[医药卫生—治疗学]